LOCUS       sequence-70727-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to 
            iRFP) in a Cre-dependent manner (DIO, double floxed inverted open 
            reading frame)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Richie CT, Whitaker LR, Whitaker KW, Necarsulmer J, Baldwin HA, 
            Zhang Y, Fortuno L, Hinkle JJ, Koivula P, Henderson MJ, Sun W, Wang 
            K, Smith JC, Pickel J, Ji N, Hope BT, Harvey BK
  TITLE     Near-infrared fluorescent protein iRFP713 as a reporter protein for 
            optogenetic vectors, a transgenic Cre-reporter rat, and other 
            neuronal studies.
  JOURNAL   J Neurosci Methods. 2017 Jun 1;284:1-14. doi: 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2.
  PUBMED    28380331
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Methods."; date: "2017-06-1"; volume: "284:1-14. doi"; pages: " 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2"
COMMENT     Sequence Label: pOTTC468 - pAAV EF1a DIO hChR2(H134R)-iRFP
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtcaggctgc ctggcgacgg atccttcagc catactagtg gcggccgctg gcacggctcc
       61 ggcctcggct tcgtcttcga cgagagtctc gacctcgatc tccgttccgc cgatgttcag
      121 tttggtggtt ttgcggatat ctccgtgaat caatatgtgc tcgtggatca ggacgcgcag
      181 gtagtgtccc aacaaccccc aacaattttt actcatcaga tcaataatcg tgtgacctac
      241 ggtggagcca tagacgctca ggacgccaaa accttcgggc cccaaaatga agagaattgg
      301 gaacataccc cagctcacga aaaacagcca tgccatgccg gtcacgacct ggcggcaccg
      361 accctttggc acagtatgat aaccctcgat atatgctttg gcggcgtgaa aaaatgtgtt
      421 cgcgccatag cacaatccaa gacaaaagaa gatgacttta acatagccgg ttgccatggc
      481 gctggtagcc ccccacacga tagtcccgat gtctgagaca aggagtccca tggttctcct
      541 gctgtagtcg ttgctcaggc cggtgaggtt gctcaggcgg ataaggatga caggacaagt
      601 gagcagccac tctgcatagc gcagccactg cacccggtgt cctgtggcaa ggtagagcat
      661 agagggattc ttaaactcaa aaaagaactc gagaatcacc ttaaccattt caatggcgca
      721 cacatagatc tcctcccagc cgcatgtaga tttccaggtt tggtaggcat agaacatcag
      781 cagcaaaatg ctgaatcctg ctgcaagcca ctgcaggaca tttgacgcgg tctgagcgcc
      841 gttcgtgccg cgagattcaa tccatccggc acagtaacat tg
//
