LOCUS       sequence-70740-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Ma AC, Lee HB, Clark KJ, Ekker SC
  TITLE     High Efficiency In Vivo Genome Engineering with a Simplified 15-RVD 
            GoldyTALEN Design.
  JOURNAL   PLoS One. 2013 May 29;8(5):e65259. doi: 
            10.1371/journal.pone.0065259. Print 2013.
  PUBMED    23734242
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-05-29"; volume: "8(5):e65259. doi"; pages: " 
            10.1371/journal.pone.0065259. Print 2013"
COMMENT     Sequence Label: pFUSB4_CCGG
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..18
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2..18
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgtaaaacga cggccagtct taagcgtctc atggcctgac cccggaccaa gtggtggcta
       61 tcgccagcca cgatggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc
      121 tgtgccagga ccatggcctg actccggacc aagtggtggc tatcgccagc cacgatggcg
      181 gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc
      241 tgaccccgga ccaagtggtg gctatcgcca gcaacaatgg cggcaagcaa gcgctcgaaa
      301 cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg
      361 tggctatcgc cagcaacaat ggcggcaagc aagcgctcga aacggtgcag cggctgttgc
      421 cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc gagacgtcta
      481 gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg ggtgacgcac
      541 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
      601 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
      661 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
      721 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
      781 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acattatga
//
