LOCUS       sequence-70741-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vitro transcription of a mRNA encoding humanized Cas9 nuclease, 
            with 3' UTRs suitable for germline expression in C. elegans, for 
            doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Chiu H, Schwartz HT, Antoshechkin I, Sternberg PW
  TITLE     Transgene-Free Genome Editing in Caenorhabditis elegans Using 
            CRISPR-Cas.
  JOURNAL   Genetics. 2013 Aug 26.
  PUBMED    23979577
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 Aug 26."
COMMENT     Sequence Label: SP6-hCas9-Ce-mRNA
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(60..80)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggcgaattgg gtaccctgca gcagatctca gggccccggt cgacgtgagc tcaggctcac
       61 accttcctct tcttcttggg gtcagccctg ctgtctccac cgagctgaga gaggtcgatt
      121 cttgtttcat agagccccgt aattgactga tgaatcagtg tggcgtccag gacctccttt
      181 gtagaggtgt accgctttct gtctatggtg gtgtcgaagt acttgaaggc tgcaggcgcg
      241 cccaagttgg tcagagtaaa caagtggata atgttttctg cctgctccct gatgggctta
      301 tccctgtgct tattgtaagc agaaagcacc ttatcgaggt tagcgtcggc gaggatcact
      361 cttttggaga attcgcttat ttgctcgatg atctcatcaa ggtagtgttt gtgttgttcc
      421 acgaacagct gcttctgctc attatcttcg ggagaccctt tgagcttttc atagtggctg
      481 gccagataca agaaattaac gtatttagag ggcagtgcca gctcgttacc tttctgcagc
      541 tcgcccgcac tagcgagcat tcgtttccgg ccgttttcaa gctcaaagag agagtacttg
      601 ggaagcttaa tgatgaggtc ttttttgacc tctttatatc ctttcgcctc gagaaagtcg
      661 atggggtttt tttcgaagct tgatcgctcc atgattgtga tgcccagcag ttccttgacg
      721 cttttgagtt ttttagactt ccctttctcc actttggcca caaccagtac actgtaagcg
      781 actgtaggag aatcgaat
//
