LOCUS       sequence-70743-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Scaffold into which clone a 20 bp targeting sequence to generate a 
            plasmid for in vitro transcription of an sgRNA using SP6 RNA 
            polymerase for use in CRISPR-Cas by RNA injection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Chiu H, Schwartz HT, Antoshechkin I, Sternberg PW
  TITLE     Transgene-Free Genome Editing in Caenorhabditis elegans Using 
            CRISPR-Cas.
  JOURNAL   Genetics. 2013 Aug 26.
  PUBMED    23979577
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 Aug 26."
COMMENT     Sequence Label: SP6-sgRNA-scaffold
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..71
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     55..71
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        91..109
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     91..108
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_RNA        116..191
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(206..225)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(207..225)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(232..249)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(232..248)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(241..263)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(527..548)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     739..758
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attaaccctc actaaaggga acaaaagctg gagctccacc gcggtggcgg ccgctctaga
       61 actagtggat cccccgggct gcaggaattc atttaggtga cactatagac ttaaggtttt
      121 agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac
      181 cgagtcggtg ctggtaccca attcgcccta tagtgagtcg tattacaatt cactggccgt
      241 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
      301 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
      361 acagttgcgc agcctgaatg gcgaatggca aattgtaagc gttaatattt tgttaaaatt
      421 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
      481 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
      541 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
      601 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
      661 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
      721 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
      781 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
      841 cg
//
