LOCUS       sequence-70758-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vitro transcription of a mRNA encoding humanized Cas9 nuclease, 
            with 3' UTRs suitable for germline expression in C. elegans, for 
            doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Chiu H, Schwartz HT, Antoshechkin I, Sternberg PW
  TITLE     Transgene-Free Genome Editing in Caenorhabditis elegans Using 
            CRISPR-Cas.
  JOURNAL   Genetics. 2013 Aug 26.
  PUBMED    23979577
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 Aug 26."
COMMENT     Sequence Label: SP6-hCas9-Ce-mRNA
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(89..100)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttccgttgtg tgatcagttt ggcgttcagc agctgccgcc aataattttt cattttcttg
       61 acaacttctt ctgaggggac gttatcactc ttccctctat ttttatcgga tcttgtcaac
      121 actttattat caatagaatc atctttgaga aaagactggg gcacgatatg atccacgtcg
      181 tagtcggaga gccgattgat gtccagttcc tgatccacgt acatgtccct gccgttctgc
      241 aggtagtaca ggtagagctt ctcattctga agctgggtgt tttcaactgg gtgttcctta
      301 aggatttggg accccagttc ttttataccc tcttcaatcc tcttcatcct ttccctactg
      361 ttcttctgtc ccttctgggt agtttggttc tctcgggcca tctcgataac gatattctcg
      421 ggcttatgcc ttcccattac tttgacgagt tcatccacga ccttaacggt ctgcagtatt
      481 ccctttttga tagctgggct acctgcaaga ttagcgatgt gctcgtgaag actgtccccc
      541 tggccagaaa cttgtgcttt ctggatgtcc tccttaaagg tgagagagtc atcatggatc
      601 aactgcatga agttccggtt ggcaaatcca tcggacttaa gaaaatccag gattgtcttt
      661 ccactctgct tgtctcggat cccattgatc agttttcttg acagccgccc ccatcctgta
      721 tatcggcgcc tcttgagctg tttcatgact ttgtcgtcga agagatgagc gtaagttttc
      781 aagcgttctt caatcatctc cctatcttca aacaacgt
//
