LOCUS       sequence-70761-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses E.coli codon optimized EYFP-Twin-Strep fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Dammeyer T, Timmis KN, Tinnefeld P
  TITLE     Broad host range vectors for expression of proteins with (Twin-) 
            Strep-tag, His-tag and engineered, export optimized yellow 
            fluorescent protein.
  JOURNAL   Microb Cell Fact. 2013 May 20;12(1):49.
  PUBMED    23687945
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-05-20"; volume: "12(1)"; pages: "49"
COMMENT     Sequence Label: pTD-C_eYFPTwinStrep
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(82..105)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(142..165)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 ttactggatc tatcaacagg agtccaagac tagtcgccag ggttttccca gtcacgacgc
       61 ggccgcaagc tttcatcatt atttttcgaa ctgcgggtgg ctccacgatc cacctcccga
      121 tccacctccg gaacctccac ctttctcgaa ctgcgggtgg ctccaagcgc tctcgagtgc
      181 ggccgctcta gatttgtaca gttcgtccat acccagggtg ataccagcag cggtaacaaa
      241 ttccagcaga accatgtggt cacgtttttc gttcgggtct ttagacagtt tagactggta
      301 agacaggtag tggttgtccg gcagcagaac cggaccgtca ccgatcgggg tgttctgctg
      361 gtagtggtca gccagctgaa cagaaccgtc ttcgatgttg tgacggattt tgaagttaac
      421 tttgataccg tttttctgtt tgtcagccat gatgtaaacg ttgtgagagt tgtagttgta
      481 ttccagtttg tgacccagga tgttaccgtc ttctttgaag tcgatacctt tcagttcgat
      541 acggttaacc agggtgtcac cttcgaattt aacttcagca cgggttttgt agttaccgtc
      601 gtctttgaag aagatggtac gttcctgaac gtaaccttcc ggcatagcag atttgaagaa
      661 gtcgtgctgt ttcatgtggt ccgggtaacg agcgaagcac tgcagaccgt aaccgaaggt
      721 ggtaaccagg gtcggccacg gaaccggcag tttaccggtg gtgcagatga atttcagggt
      781 cagtttaccg taggtagcgt caccttcacc ttcaccagaa acagagaatt tgtgaccgtt
      841 aacgtcaccg tccagttcaa ccaggatcgg aacaacaccg gtgaacagtt cttcaccttt
      901 agaaacgatc gaattcggat ccatatgtat atctccttct taaagttaac aattcgcgcg
      961 gccgcggcct agggtgtg
//
