LOCUS       sequence-7077-ex         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Zhou Q, Snipas S, Orth K, Muzio M, Dixit VM, Salvesen GS
  TITLE     Target protease specificity of the viral serpin CrmA. Analysis of 
            five caspases.
  JOURNAL   J Biol Chem. 1997 Mar 21. 272(12):7797-800.
  PUBMED    9065443
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1997 Mar 21. 272(12):7797-800."
COMMENT     Sequence Label: pET23b-Casp6-His
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             9..31
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             927..944
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gaaataattt tgtttaactt taagaaggag atatacatat ggctagctcg gcctcggggc
       61 tccgcagggg gcacccggca ggtggggaag aaaacatgac agaaacagat gccttctata
      121 aaagagaaat gtttgatccg gcagaaaagt acaaaatgga ccacaggagg agaggaattg
      181 ctttaatctt caatcatgag aggttctttt ggcacttaac actgccagaa aggcggggca
      241 cctgcgcaga tagagacaat cttacccgca ggttttcaga tctaggattt gaagtgaaat
      301 gctttaatga tcttaaagca gaagaactac tgctcaaaat tcatgaggtg tcaactgtta
      361 gccacgcaga tgccgattgc tttgtgtgtg tcttcctgag ccatggcgaa ggcaatcaca
      421 tttatgcata tgatgctaaa atcgaaattc agacattaac tggcttgttc aaaggagaca
      481 agtgtcacag cctggttgga aaacccaaga tatttatcat tcaggcatgt cggggaaacc
      541 agcacgatgt gccagtcatt cctttggatg tagtagataa tcagacagag aagttggaca
      601 ccaacataac tgaggtggat gcagcctccg tttacacgct gcctgctgga gctgacttcc
      661 tcatgtgtta ctctgttgca gaaggatatt attctcaccg ggaaactgtg aacggctcat
      721 ggtacattca agatttgtgt gagatgttgg gaaaatatgg ctcctcctta gagttcacag
      781 aactcctcac actggtgaac aggaaagttt ctcagcgccg agtggacttt tgcaaagacc
      841 caagtgcaat tggaaagaag caggttccct gttttgcctc aatgctaact aaaaagctgc
      901 atttctttcc aaaatctaat ctcgagcacc accaccacca ccactgagat ccggctgcta
      961 acaaagcccg aaaggaagct gagttggc
//
