LOCUS       sequence-70773-e         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses P.putida codon optimized super-folder sfYFP-Twin-Strep 
            fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Dammeyer T, Timmis KN, Tinnefeld P
  TITLE     Broad host range vectors for expression of proteins with (Twin-) 
            Strep-tag, His-tag and engineered, export optimized yellow 
            fluorescent protein.
  JOURNAL   Microb Cell Fact. 2013 May 20;12(1):49.
  PUBMED    23687945
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-05-20"; volume: "12(1)"; pages: "49"
COMMENT     Sequence Label: pTD-C_sfYFPTwinStrep
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(82..105)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(142..165)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 ttactggatc tatcaacagg agtccaagac tagtcgccag ggttttccca gtcacgacgc
       61 ggccgcaagc tttcatcatt atttttcgaa ctgcgggtgg ctccacgatc cacctcccga
      121 tccacctccg gaacctccac ctttctcgaa ctgcgggtgg ctccaagcgc tctcgagtgc
      181 ggccgctcta gacttgtaca gttcgtccat gcccagggtg atgccggcgg cggtcacaaa
      241 ttccagcagc accatgtggt cgcgcttttc gttcgggtcc ttcgacagct tcgactggta
      301 cgacaggtag tggttgtccg gcagcagcac cgggccgtcg ccgatcgggg tgttctgctg
      361 gtagtggtcg gccagctgca cgccgccgtc ttccacgttg tggcggatct tgaagttggc
      421 cttgatgccg ttcttctgct tgtcggcggt gatgtacacg ttgtgcgagt tgaagttgta
      481 ttccagcttg tggcccagga tgttgccgtc ttccttgaag tcgatgccct tcagttcgat
      541 gcggttcacc agggtgtcgc cttcgaactt cacttcggcg cgggtcttgt aggtgccgtc
      601 gtccttgaac gagatggtgc gttcctgcac gtagccttcc ggcatggccg acttgaagaa
      661 gtcgtgctgc ttcatgtggt ccgggtagcg ggcgaagcac tgcaggccgt agcccagggt
      721 ggtcaccagg gtcggccacg gcaccggcag cttgccggtg gtgcagatga acttcagggt
      781 cagcttgccg ttggtggcgt cgccttcgcc ttcgcccgac acgcggaact tgtggtcgtt
      841 cacgtcgccg tccagttcca ccaggatcgg caccacgccg gtgaacagtt cttcgccctt
      901 cgacaccgaa ttcggatcca tatgtatatc tccttcttaa agttaacaat tcgcgcg
//
