LOCUS       sequence-70776-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Nguyen AW, Daugherty PS
  TITLE     Evolutionary optimization of fluorescent proteins for intracellular 
            FRET.
  JOURNAL   Nat Biotechnol. 2005 Mar;23(3):355-60. doi: 10.1038/nbt1066
  PUBMED    15696158
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2005-03"; volume: "23"; issue: "3"; pages: 
            "355-60"
COMMENT     Sequence Label: pCyPet-His
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(71..88)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(89..802)
                     /codon_start=1
                     /product="cyan fluorescent protein optimized for FRET with 
                     YPet (Nguyen and Daugherty, 2005)"
                     /label=CyPet
                     /translation="MSKGEELFGGIVPILVELEGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSVMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKA
                     NFKARHNITDGSVQLADHYQQNTPIGDGPVILPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 ccaaacagcc agcttgcatg cctgcaggtc gactctagag gatccccggg taccggccac
       61 cttggcctta gtggtggtgg tggtggtgtt tgtacaattc atccatacca tgggtaatac
      121 cagcagcagt aacaaattcg agcaagacca tgtggtctct cttttcgttt ggatctttag
      181 ataaggcaga ttgagtggat aagtaatggt tgtctggcaa gatgactgga ccatcaccaa
      241 ttggagtatt ttgttgataa tggtcagcta attgaacaga accatcggta atgttgtgtc
      301 tggctttgaa gttagctttg ataccattct tttgtttgtc agcggtgatg taaacattgt
      361 gagagatata gttgtattcc aatttgtgac ctaaaatgtt accatcttct ttaaaatcaa
      421 taccttttaa ttcgattcta ttaactaagg tatcaccttc aaacttgact tcagctctgg
      481 tcttgtagtt accgtcatct ttgaaaaaaa tagttctttc ttgaacataa ccttctggca
      541 tgacagactt gaaaaagtca tgttgtttca tatgatctgg gtatctagaa aaacattgaa
      601 caccccaagt cagagtagtg actaaggttg gccatggaac tggcaattta ccagtagtac
      661 aaataaattt taaggtcaat ttaccgtacg tagcatcacc ttcaccttca ccggagacag
      721 aaaatttgtg accattaaca tcaccctcta attcaactaa aattgggacg ataccgccga
      781 ataattcttc acctttagac atcgtttcac tcctggcctt cgtggccgag ctcgaattcg
      841 ctagcccaaa aaaaacgggt atggagaaac agtagagagt tgcgataa
//
