LOCUS       sequence-70785-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Ohta T, Xiong Y
  TITLE     Phosphorylation- and Skp1-independent in vitro ubiquitination of 
            E2F1 by multiple ROC-cullin ligases.
  JOURNAL   Cancer Res. 2001 Feb 15. 61(4):1347-53.
  PUBMED    11245432
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2001 Feb 15. 61(4):1347-53."
COMMENT     Sequence Label: pcDNA3-myc-Skp2
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        34..52
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             68..97
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
       61 agctatggag caaaagctca tctcagagga agatctggaa ttcccgggga tccccatgga
      121 cgtatttaaa actcccgggc ctgcggacgc tatgcacagg aagcacctcc aggagattcc
      181 agacctgagt agcaacgttg ccaccagctt cacgtgggga tgggattcca gcaagacttc
      241 tgaactgctg tcaggcatgg gggtctccgc cctggagaaa gaggagcccg acagtgagaa
      301 catcccccag gaactgctct caaacctggg ccacccggag agccccccac ggaaacggct
      361 gaagagcaaa gggagtgaca aagactttgt gattgtccgc aggcctaagc taaatcgaga
      421 gaactttcca ggtgtttcat gggactccct tccggatgag ctgctcttgg gaatcttttc
      481 ctgtctgtgc ctccctgagc tgctaaaggt ctctggtgtt tgtaagaggt ggtatcgcct
      541 agcgtctgat gagtctctat ggcagacctt agacctcaca ggtaaaaatc tgcacccgga
      601 tgtgactggt cggttgctgt ctcaaggggt gattgccttc cgctgcccac gatcatttat
      661 ggaccaacca ttggctgaac atttcagccc ttttcgtgta cagcacatgg acctatcgaa
      721 ctcagttata gaagtgtcca ccctccacgg catactgtct cagtgttcca agttgcagaa
      781 tctaagcctg gaaggcctgc ggctttcgga tcccattgtc aatactctcg caaaaaactc
      841 aaatttagtg cgacttaacc tttctgggtg ttctggattc tctgaatttg ccc
//
