LOCUS       sequence-70791-e        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to 
            purify specific genomic regions of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Fujita T, Fujii H
  TITLE     Efficient isolation of specific genomic regions and identification 
            of associated proteins by engineered DNA-binding molecule-mediated 
            chromatin immunoprecipitation (enChIP) using CRISPR.
  JOURNAL   Biochem Biophys Res Commun. 2013 Sep 13;439(1):132-6. doi: 
            10.1016/j.bbrc.2013.08.013. Epub 2013 Aug 11.
  PUBMED    23942116
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2013.08.013"; journalName: "Biochem Biophys Res 
            Commun"; date: "2013-09-13- 13"; volume: "439"; issue: "1"; pages: 
            "132-6"
COMMENT     Sequence Label: 3xFLAG-dCas9/pCMV-7.1
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(99..110)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 attatcgaac ttccgttgtg tgatcagttt ggcgttcagc agctgccgcc aataattttt
       61 cattttcttg acaacttctt ctgaggggac gttatcactc ttccctctat ttttatcgga
      121 tcttgtcaac actttattat caatagaatc atctttgaga aaagactggg gcacgatggc
      181 atccacgtcg tagtcggaga gccgattgat gtccagttcc tgatccacgt acatgtccct
      241 gccgttctgc aggtagtaca ggtagagctt ctcattctga agctgggtgt tttcaactgg
      301 gtgttcctta aggatttggg accccagttc ttttataccc tcttcaatcc tcttcatcct
      361 ttccctactg ttcttctgtc ccttctgggt agtttggttc tctcgggcca tctcgataac
      421 gatattctcg ggcttatgcc ttcccattac tttgacgagt tcatccacga ccttaacggt
      481 ctgcagtatt ccctttttga tagctgggct acctgcaaga ttagcgatgt gctcgtgaag
      541 actgtccccc tggccagaaa cttgtgcttt ctggatgtcc tccttaaagg tgagagagtc
      601 atcatggatc aactgcatga agttcctgtt ggcaaatcca tcggacttaa gaaaatccag
      661 gattgtcttt ccactctgct tgtctcggat cccattgatc agttttcttg acagccgccc
      721 ccatcctgta tatcggcgcc tcttgagctg tttcatgact ttgtcgtcga agagatgagc
      781 gtaagttttc aagcgttctt caatcatctc cctatcttca aacaacgtaa gggtgaggac
      841 aatgtcctca agaatgtcct cgttctcctc attgtccagg aagtccttgt ctttaatgat
      901 tttcaggaga tcgtgatacg ttcccaggga tgcgttgaag cgatcctcca ctccgctgat
      961 ttcaacagag tcgaaacatt caatcttttt gaaatagtct tctttga
//
