LOCUS       sequence-70793-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Aranko AS, Oeemig JS, Kajander T, Iwai H
  TITLE     Intermolecular domain swapping induces intein-mediated protein 
            alternative splicing.
  JOURNAL   Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320.
  PUBMED    23974115
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320."
COMMENT     Sequence Label: pSABAD332
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             61..83
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             415..432
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(472..489)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      642..728
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgctttttat cgcaactctc tactgtttct ccatacccgt ttttttgggc tagaaataat
       61 tttgtttaac tttaagaagg agatatacat atgagaataa tatccaaagg agaacttgaa
      121 ttccatgagg tttcctcggt aaggataata gattacaata actgggtcta cgaccttgta
      181 attccggaaa cccacaactt catagctccc aatggacttg ttctccataa tactcagcta
      241 ggtacccagt acaaacttat cctgaacggt aaaaccctga aaggtgaaac caccaccgaa
      301 gctgttgacg ctgctaccgc ggaaaaagtt ttcaaacagt acgctaacga caacggtgtt
      361 gacggtgaat ggacctacga cgacgctacc aaaacctaca ccgtaacgga attccatcat
      421 catcatcatc attaagcttg gctgttttgg cggatgagag aagattttca gcctgataca
      481 gattaaatca gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc
      541 ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag
      601 tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc
      661 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
      721 ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgg
//
