LOCUS       sequence-70794-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Aranko AS, Oeemig JS, Kajander T, Iwai H
  TITLE     Intermolecular domain swapping induces intein-mediated protein 
            alternative splicing.
  JOURNAL   Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320.
  PUBMED    23974115
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320."
COMMENT     Sequence Label: pSABAD250
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             62..84
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             392..409
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(449..466)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      619..705
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg ctagaaataa
       61 ttttgtttaa ctttaagaag gagatataca tatgagtgat atttattggg atgaaatagt
      121 ttcaattgaa tatagtggtg aggaagaagt gtttgacctc accgttcctg gtttgcataa
      181 ttttgttgca aataatatta ttgttcacaa ttccattggt acccagtaca aacttatcct
      241 gaacggtaaa accctgaaag gtgaaaccac caccgaagct gttgacgctg ctaccgcgga
      301 aaaagttttc aaacagtacg ctaacgacaa cggtgttgac ggtgaatgga cctacgacga
      361 cgctaccaaa acctacaccg taacggaatt ccatcatcat catcatcatt aagcttggct
      421 gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg
      481 gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc
      541 cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag
      601 tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      661 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat
      721 ttgaacgttg cgaagcaacg gccgg
//
