LOCUS       sequence-7080-ex        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Zhou Q, Snipas S, Orth K, Muzio M, Dixit VM, Salvesen GS
  TITLE     Target protease specificity of the viral serpin CrmA. Analysis of 
            five caspases.
  JOURNAL   J Biol Chem. 1997 Mar 21. 272(12):7797-800.
  PUBMED    9065443
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1997 Mar 21. 272(12):7797-800."
COMMENT     Sequence Label: pET23b-Casp7 C186A-His
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             958..975
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1028..1046)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tctagaaata attttgttta actttaagaa ggagatatac atatggcaga tgatcagggc
       61 tgtattgaag agcagggggt tgaggattca gcaaatgaag attcagtgga tgctaagcca
      121 gaccggtcct cgtttgtacc gtccctcttc agtaagaaga agaaaaatgt caccatgcga
      181 tccatcaaga ccacccggga ccgagtgcct acatatcagt acaacatgaa ttttgaaaag
      241 ctgggcaaat gcatcataat aaacaacaag aactttgata aagtgacagg tatgggcgtt
      301 cgaaacggaa cagacaaaga tgccgaggcg ctcttcaagt gcttccgaag cctgggtttt
      361 gacgtgattg tctataatga ctgctcttgt gccaagatgc aagatctgct taaaaaagct
      421 tctgaagagg accatacaaa tgccgcctgc ttcgcctgca tcctcttaag ccatggagaa
      481 gaaaatgtaa tttatgggaa agatggtgtc acaccaataa aggatttgac agcccacttt
      541 aggggggata gatgcaaaac ccttttagag aaacccaaac tcttcttcat tcaggctgcc
      601 cgagggaccg agcttgatga tggcatccag gccgactcgg ggcccatcaa tgacacagat
      661 gctaatcctc gatacaagat cccagtggaa gctgacttcc tcttcgccta ttccacggtt
      721 ccaggctatt actcgtggag gagcccagga agaggctcct ggtttgtgca agccctctgc
      781 tccatcctgg aggagcacgg aaaagacctg gaaatcatgc agatcctcac cagggtgaat
      841 gacagagttg ccaggcactt tgagtctcag tctgatgacc cacacttcca tgagaagaag
      901 cagatcccct gtgtggtctc catgctcacc aaggaactct acttcagtca actcgagcac
      961 caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct
     1021 gctgccaccg ctgagcaata actagcataa ccc
//
