LOCUS       sequence-70803-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses P.putida codon optimized super-folder sfYFP-Twin-Strep 
            fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Dammeyer T, Timmis KN, Tinnefeld P
  TITLE     Broad host range vectors for expression of proteins with (Twin-) 
            Strep-tag, His-tag and engineered, export optimized yellow 
            fluorescent protein.
  JOURNAL   Microb Cell Fact. 2013 May 20;12(1):49.
  PUBMED    23687945
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-05-20"; volume: "12(1)"; pages: "49"
COMMENT     Sequence Label: pTD-C_sfYFPTwinStrep
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(342..347,348..364,365..371)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    379..395
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
       61 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      121 attaatgtaa gttagcgcga attgatctgg tttgacagct tatcatcgac tgcacggtgc
      181 accaatgctt ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa
      241 atcactgcat aattcgtgtc gctcaaggcg cactcccgtt ctggataatg ttttttgcgc
      301 cgacatcata acggttctgg caaatattct gaaatgagct gttgacaatt aatcatccgg
      361 ctcgtataat gtgtggaatt gtgagcggat aacaatttca caccctaggc cgcggccgcg
      421 cgaattgtta actttaagaa ggagatatac atatggatcc gaattcggtg tcgaagggcg
      481 aagaactgtt caccggcgtg gtgccgatcc tggtggaact ggacggcgac gtgaacggcc
      541 acaagttccg cgtgtcgggc gaaggcgaag gcgacgccac caacggcaag ctgaccctga
      601 agttcatctg caccaccggc aagctgccgg tgccgtggcc gaccctggtg accaccctgg
      661 gctacggcct gcagtgcttc gcccgctacc cggaccacat gaagcagcac gacttcttca
      721 agtcggccat gccggaaggc tacgtgcagg aacgcaccat ctcgttcaag gacgacggca
      781 cctacaagac ccgcgccgaa gtgaagttcg aaggcgacac cctggtgaac cgcatcgaac
      841 tgaagggcat cgacttcaag gaagacggca acatcctggg ccacaagctg gaatacaact
      901 tcaactcgca caacgtgtac atcaccgccg acaagcagaa gaacggcatc a
//
