LOCUS       sequence-70805-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Aranko AS, Oeemig JS, Kajander T, Iwai H
  TITLE     Intermolecular domain swapping induces intein-mediated protein 
            alternative splicing.
  JOURNAL   Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320.
  PUBMED    23974115
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320."
COMMENT     Sequence Label: pSABAD544
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             61..83
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(124..152)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgctttttat cgcaactctc tactgtttct ccatacccgt ttttttgggc tagaaataat
       61 tttgtttaac tttaagaagg agatatacac atggctagca aaggagaaga acttttcact
      121 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
      181 cgcggagagg gtgaaggtga tgctacaaac ggaaaactta cccttaaatt tatttgcact
      241 actggaaaac tacctgttcc atggccaaca ctagttacta ctttatgttt aagctatgaa
      301 acggaaatat tgacagtaga atatggatta ttaccgattg gtaaaattgt agaaaagcgc
      361 atcgaatgta ctgtttatag cgttgataat aatggaaata tttatacaca acctgtagca
      421 caatggcacg atcgcggaga acaagaggtg tttgagtatt gtttggaaga tggttcattg
      481 attcgggcaa caaaagacca taagtttatg actgttgatg gtcaaatgtt gccaattgat
      541 gaaatatttg aacgtgaatt ggatttgatg cgggttgata atttgccgaa tatcaaaata
      601 gccacacgta aatatttagg caaacaaaat gtctatgaca ttggagttga gcgcgaccat
      661 aattttgcac tcaaaaatgg cttcatagcc gcgggttgtt atggtgttca atgcttttct
      721 agatatccgg atcatatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat
      781 gtacaggaac gcactatatc tttcaaagat gacgggaact acaagacgcg tgctgaagtc
      841 aagtttgaag gtgataccct tgttaatcgt atcgagttaa aaggtattga ttttaaagaa
//
