LOCUS       sequence-7081-ex         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Zhou Q, Snipas S, Orth K, Muzio M, Dixit VM, Salvesen GS
  TITLE     Target protease specificity of the viral serpin CrmA. Analysis of 
            five caspases.
  JOURNAL   J Biol Chem. 1997 Mar 21. 272(12):7797-800.
  PUBMED    9065443
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1997 Mar 21. 272(12):7797-800."
COMMENT     Sequence Label: pET15b-Casp8 delta DED
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..83
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gtttaacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat catcacagca
       61 gcggcctggt gccgcgcggc agccatatga gtgaatcaca gactttggac aaagtttacc
      121 aaatgaaaag caaacctcgg ggatactgtc tgatcatcaa caatcacaat tttgcaaaag
      181 cacgggagaa agtgcccaaa cttcacagca ttagggacag gaatggaaca cacttggatg
      241 caggggcttt gaccacgacc tttgaagagc ttcattttga gatcaagccc cacgatgact
      301 gcacagtaga gcaaatctat gagattttga aaatctacca actcatggac cacagtaaca
      361 tggactgctt catctgctgt atcctctccc atggagacaa gggcatcatc tatggcactg
      421 atggacagga gccccccatc tatgagctga catctcagtt cactggtttg aagtgccctt
      481 cccttgctgg aaaacccaaa gtgtttttta ttcaggcttg tcagggggat aactaccaga
      541 aaggtatacc tgttgagact gattcagagg agcaacccta tttagaaatg gatttatcat
      601 cacctcaaac gagatatatc ccggatgagg ctgactttct gctggggatg gccactgtga
      661 ataactgtgt ttcctaccga aaccctgcag agggaacctg gtacatccag tcactttgcc
      721 agagcctgag agagcgatgt cgcgatgata ttctcaccat cctgactgaa gtgaactatg
      781 aagtaagcaa caaggatgac aagaaaaaca tggggaaaca gatgcctcag cctactttca
      841 cactaagaaa aaaacttgtc ttcccttcta gagatatcgt cgacagatct ctcgagtgag
      901 agaagatttt cagcctgata cagatt
//
