LOCUS       sequence-7082-ex         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Zhou Q, Snipas S, Orth K, Muzio M, Dixit VM, Salvesen GS
  TITLE     Target protease specificity of the viral serpin CrmA. Analysis of 
            five caspases.
  JOURNAL   J Biol Chem. 1997 Mar 21. 272(12):7797-800.
  PUBMED    9065443
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1997 Mar 21. 272(12):7797-800."
COMMENT     Sequence Label: pET15b-Casp8 delta DED C360A
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..46
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             56..73
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aagaaggaga tataccatgg gcagcagcca tcatcatcat catcacagca gcggcctggt
       61 gccgcgcggc agccatatga gtgaatcaca gactttggac aaagtttacc aaatgaaaag
      121 caaacctcgg ggatactgtc tgatcatcaa caatcacaat tttgcaaaag cacgggagaa
      181 agtgcccaaa cttcacagca ttagggacag gaatggaaca cacttggatg caggggcttt
      241 gaccacgacc tttgaagagc ttcattttga gatcaagccc cacgatgact gcacagtaga
      301 gcaaatctat gagattttga aaatctacca actcatggac cacagtaaca tggactgctt
      361 catctgctgt atcctctccc atggagacaa gggcatcatc tatggcactg atggacagga
      421 ggcccccatc tatgagctga catctcagtt cactggtttg aagtgccctt cccttgctgg
      481 aaaacccaaa gtgtttttta ttcaggctgc tcagggggat aactaccaga aaggtatacc
      541 tgttgagact gattcagagg agcaacccta tttagaaatg gatttatcat cacctcaaac
      601 gagatatatc ccggatgagg ctgactttct gctggggatg gccactgtga ataactgtgt
      661 ttcctaccga aaccctgcag agggaacctg gtacatccag tcactttgcc agagcctgag
      721 agagcgatgt cctcgaggcg atgatattct caccatcctg actgaagtga actatgaagt
      781 aagcaacaag gatgacaaga aaaacatggg gaaacagatg cctcagccta ctttcacact
      841 aagaaaaaaa cttgtcttcc cttctgattg aggatccggc tgctaacaaa gcccgaaagg
//
