LOCUS       sequence-70820-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Ma AC, Lee HB, Clark KJ, Ekker SC
  TITLE     High Efficiency In Vivo Genome Engineering with a Simplified 15-RVD 
            GoldyTALEN Design.
  JOURNAL   PLoS One. 2013 May 29;8(5):e65259. doi: 
            10.1371/journal.pone.0065259. Print 2013.
  PUBMED    23734242
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-05-29"; volume: "8(5):e65259. doi"; pages: " 
            10.1371/journal.pone.0065259. Print 2013"
COMMENT     Sequence Label: pFUSB4_CCCG
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..21
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5..21
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgttgtaaaa cgacggccag tcttaagcgt ctcatggcct gaccccggac caagtggtgg
       61 ctatcgccag ccacgatggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg
      121 tgctgtgcca ggaccatggc ctgactccgg accaagtggt ggctatcgcc agccacgatg
      181 gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg
      241 gcctgactcc ggaccaagtg gtggctatcg ccagccacga tggcggcaag caagcgctcg
      301 aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc ccggaccaag
      361 tggtggctat cgccagcaac aatggcggca agcaagcgct cgaaacggtg cagcggctgt
      421 tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct atcgagacgt
      481 ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg
      541 cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa
      601 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
      661 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
      721 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
      781 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacattat
      841 gagggaagcg gtgatcgccg aagtatcgac tcaactatca ga
//
