LOCUS       sequence-70821-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Aranko AS, Oeemig JS, Kajander T, Iwai H
  TITLE     Intermolecular domain swapping induces intein-mediated protein 
            alternative splicing.
  JOURNAL   Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320.
  PUBMED    23974115
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320."
COMMENT     Sequence Label: pHYDuet216A
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             882..926
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 aataattttg tttaacttta ataaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtg ccgcgcggca gcctaggaca gtacaaactt atcctgaacg
      121 gtaaaaccct gaaaggtgaa accaccaccg aagctgttga cgctgctacc gcggaaaaag
      181 ttttcaaaca gtacgctaac gacaacggtg ttgacggtga atggacctac gacgacgcta
      241 ccaaaaccta caccgtaacg gaaggatcct gtttaagcta tgaaacggaa atattgacag
      301 tagaatatgg attattaccg attggtaaaa ttgtagaaaa gcgcatcgaa tgtactgttt
      361 atagcgttga taataatgga aatatttata cacaacctgt agcacaatgg cacgatcgcg
      421 gagaacaaga ggtgtttgag tattgtttgg aagatggttc attgattcgg gcaacaaaag
      481 accataagtt tatggtcgac ggtcaaatgt tgccaattga tgaaatattt gaacgtgaat
      541 tggatttgat gcggaacccc gggatcaaaa tagccacacg taaatattta ggcaaacaaa
      601 atgtctatga cattggagtt gagcgcgacc ataattttgc actcaaaaat ggcttcatag
      661 cttctaattg tttcaatggt acccagtaca aacttatcct gaacggtaaa accctgaaag
      721 gtgaaaccac caccgaagct gttgacgctg ctaccgcgga aaaagttttc aaacagtacg
      781 ctaacgacaa cggtgttgac ggtgaatgga cctacgacga cgctaccaaa acctacaccg
      841 taacggaata agcttggctg ttttggcggc tcgagtctgg taaagaaacc gctgctgcga
      901 aatttgaacg ccagcacatg gactcgtcta ctagcgcagc ttaattaa
//
