LOCUS       sequence-70824-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Aranko AS, Oeemig JS, Kajander T, Iwai H
  TITLE     Intermolecular domain swapping induces intein-mediated protein 
            alternative splicing.
  JOURNAL   Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320.
  PUBMED    23974115
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320."
COMMENT     Sequence Label: pSARSF505
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(98..126)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ataattttgt ttaactttaa taaggagata taccatgggc agcagccatc atcatcatca
       61 tcacagcact agcaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      121 agatggtgat gttaatgggc acaaattttc tgtccgcgga gagggtgaag gtgatgctac
      181 aaacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
      241 aacactagtt actactttat gtttaagcta tgaaacggaa atattgacag tagaatatgg
      301 attattaccg attggtaaaa ttgtagaaaa gcgcatcgaa tgtactgttt atagcgttga
      361 taataatgga aatatttata cacaacctgt agcacaatgg cacgatcgcg gagaacaaga
      421 ggtgtttgag tattgtttgg aagatggttc attgattcgg gcaacaaaag accataagtt
      481 tatgactgtt gatggtcaaa tgttgccaat tgatgaaata tttgaacgtg aattggattt
      541 gatgcgggtt gataatttgc cgaatatcaa aatagccaca cgtaaatatt taggcaaaca
      601 aaatgtctat gacattggag ttgagcgcga ccataatttt gcactcaaaa atggcttcat
      661 agcttctaat tgttatggtg ttcaatgctt ttctagatat ccggatcata tgaaacggca
      721 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaacgcacta tatctttcaa
      781 agatgacggg aactacaaga cgcgtgctga agtcaagttt gaaggtgata cccttgttaa
      841 tcgtatcgag ttaaaaggta ttgatttta
//
