LOCUS       sequence-70825-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Aranko AS, Oeemig JS, Kajander T, Iwai H
  TITLE     Intermolecular domain swapping induces intein-mediated protein 
            alternative splicing.
  JOURNAL   Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320.
  PUBMED    23974115
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320."
COMMENT     Sequence Label: pHYRSF236
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..357
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             797..841
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(879..897)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaataag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcactagtg actcagaagt caatcaagaa gctaagccag aggtcaagcc agaagtcaag
      121 cctgagactc acatcaattt aaaggtgtcc gatggatctt cagaaatctt ctttaagatc
      181 aaaaagacca ctcctttaag aaggctgatg gaagcgttcg ctaaaagaca gggtaaggaa
      241 atggactcct taagattctt gtacgacggt attagaattc aagctgatca gacccctgaa
      301 gatttggaca tggaggataa cgatattatt gagactcaca gagaacagat tggtggatcc
      361 ggaggagctc taagctatga aacggaaata ttgacagtag aatatggatt attaccgatt
      421 ggtaaaattg tagaaaagcg catcgaatgt actgtttata gcgttgataa taatggaaat
      481 atttatacac aacctgtagc acaatggcac gatcgcggag aacaagaggt gtttgagtat
      541 tgtttggaag atggttcatt gattcgggca acaaaagacc ataagtttat ggtcgacggt
      601 caaatgttgc caattgatga aatatttgaa cgtgaattgg atttgatgcg gaaccccggg
      661 atcaaaatag ccacacgtaa atatttaggc aaacaaaatg tctatgacat tggagttgag
      721 cgcgaccata attttgcact caaaaatggc ttcatagctt ctaatgccta agcttgcggc
      781 cgcactcgag tctggtaaag aaaccgctgc tgcgaaattt gaacgccagc acatggactc
      841 gtctactagc gcagcttaat taacctaggc tgctgccacc gctgagcaat aactagcata
      901 accccttggg gcctctaaac gggtcttgag gggtt
//
