LOCUS       sequence-70827-e         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Aranko AS, Oeemig JS, Kajander T, Iwai H
  TITLE     Intermolecular domain swapping induces intein-mediated protein 
            alternative splicing.
  JOURNAL   Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320.
  PUBMED    23974115
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320."
COMMENT     Sequence Label: pSADuet502
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..91
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ataattttgt ttaactttaa taaggagata taccatgggc agcagccatc atcatcatca
       61 tcacagcagc ggcctggtgc cgcgcggcag cctaggacag tacaaactta tcctgaacgg
      121 taaaaccctg aaaggtgaaa ccaccaccga agctgttgac gctgctaccg cggaaaaagt
      181 tttcaaacag tacgctaacg acaacggtgt tgacggtgaa tggacctacg acgacgctac
      241 caaaacctac accgtaacgg aaggatccgg ttgtttagca ggcgatagtc tggtgacatt
      301 agtagatagc ggactgcaag tgccaattaa ggaattagta ggtaaatctg gttttgcagt
      361 ttgggcattg aacgaagcta caatgcagct agaaaaggca attgttagca atgctttttc
      421 gactggtata aagcctctgt ttactttgac aactcgattg gggcggaaaa tccgtgccac
      481 aggtaatcac aagtttttaa caattaatgg ctggaaaaga cttgatgaat taactcctaa
      541 agaacatctc tgtttaccaa gaaatctccc cagttctggt aaacaaacta tgacttatgc
      601 cgaagttgca ttattagggc atttaattgg cgacggttgt acattgccac gtcatgccat
      661 acagtacact accagagaaa tagatttagc tcagaatgtt gctttcttgg caacagaagt
      721 ttttggagac tcaattgttc ctagaatttc acctgaacgt gagtggtatc aagtttactt
      781 atccgcagca cagcactgag agatcccctc ataatttccc caaagcgtaa ccatgtgtga
      841 ataaattttg agctagtagg gttgcagcca cgagtaagtc ttcccttgtt attgtgtagc
      901 cagaatgccg caaaacttcc atgcctaagc gaactgttga gagtacgttt cgatttctga
      961 ctgtgttagc ctgga
//
