LOCUS       sequence-70829-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Aranko AS, Oeemig JS, Kajander T, Iwai H
  TITLE     Intermolecular domain swapping induces intein-mediated protein 
            alternative splicing.
  JOURNAL   Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320.
  PUBMED    23974115
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320."
COMMENT     Sequence Label: pSARSF534-3
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..91
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     664..684
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             729..773
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(811..829)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      825..872
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ataattttgt ttaactttaa taaggagata taccatgggc agcagccatc atcatcatca
       61 tcacagcagc ggcctggtgc cgcgcggcag ccatatgatc aaaatagcca cacgtaaata
      121 tttaggcaaa caaaatgtct atgacattgg agttgagcgc gaccataatt ttgcactcaa
      181 aaatggcttc atagcttcta attgttatgg tgttcaatgc ttttctagat atccggatca
      241 tatgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac
      301 tatatctttc aaagatgacg ggaactacaa gacgcgtgct gaagtcaagt ttgaaggtga
      361 tacccttgtt aatcgtatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
      421 cggacacaaa ctcgagtaca actataactc acacaatgta tacatcacgg cagacaaaca
      481 aaagaatgga atcaaagcta acttcaaaat tcgccacaac attgaagatg gatctgttca
      541 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
      601 caaccattac ctgtcgacac aatctgccct ttcgaaagat cccaacgaaa agcgtgacca
      661 catggtcctt cttgagtttg taactgctgc tgggttgact taagcttgcg gccgcactcg
      721 agtctggtaa agaaaccgct gctgcgaaat ttgaacgcca gcacatggac tcgtctacta
      781 gcgcagctta attaacctag gctgctgcca ccgctgagca ataactagca taaccccttg
      841 gggcctctaa acgggtcttg aggggttttt tgctga
//
