LOCUS       sequence-7083-ex        1373 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Stennicke HR, Deveraux QL, Humke EW, Reed JC, Dixit VM, Salvesen GS
  TITLE     Caspase-9 can be activated without proteolytic processing.
  JOURNAL   J Biol Chem. 1999 Mar 26. 274(13):8359-62.
  PUBMED    10085063
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1999 Mar 26. 274(13):8359-62."
COMMENT     Sequence Label: pET23b-Casp9-His
FEATURES             Location/Qualifiers
     source          1..1373
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             complement(760..771)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             1279..1296
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1309..1326
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atggacgaag cggatcggcg gctcctgcgg
       61 cggtgccggc tgcggctggt ggaagagctg caggtggacc agctctggga cgtcctgctg
      121 agccgcgagc tgttcaggcc ccatatgatc gaggacatcc agcgggcagg ctctggatct
      181 cggcgggatc aggccaggca gctgatcata gatctggaga ctcgagggag tcaggctctt
      241 cctttgttca tctcctgctt agaggacaca ggccaggaca tgctggcttc gtttctgcga
      301 actaacaggc aagcagcaaa gttgtcgaag ccaaccctag aaaaccttac cccagtggtg
      361 ctcagaccag agattcgcaa accagaggtt ctcagaccgg aaacacccag accagtggac
      421 attggttctg gaggattcgg tgatgtcggt gctcttgaga gtttgagggg aaatgcagat
      481 ttggcttaca tcctgagcat ggagccctgt ggccactgcc tcattatcaa caatgtgaac
      541 ttctgccgtg agtccgggct ccgcacccgc actggctcca acatcgactg tgagaagttg
      601 cggcgtcgct tctcctcgct gcatttcatg gtggaggtga agggcgacct gactgccaag
      661 aaaatggtgc tggctttgct ggagctggcg cggcaggacc acggtgctct ggactgctgc
      721 gtggtggtca ttctctctca cggctgtcag gccagccacc tgcagttccc aggggctgtc
      781 tacggcacag atggatgccc tgtgtcggtc gagaagattg tgaacatctt caatgggacc
      841 agctgcccca gcctgggagg gaagcccaag ctctttttca tccaggcctg tggtggggag
      901 cagaaagacc atgggtttga ggtggcctcc acttcccctg aagacgagtc ccctggcagt
      961 aaccccgagc cagatgccac cccgttccag gaaggtttga ggaccttcga ccagctggac
     1021 gccatatcta gtttgcccac acccagtgac atctttgtgt cctactctac tttcccaggt
     1081 tttgtttcct ggagggaccc caagagtggc tcctggtacg ttgagaccct ggacgacatc
     1141 tttgagcagt gggctcactc tgaagacctg cagtccctcc tgcttagggt cgctaatgct
     1201 gtttcggtga aagggattta taaacagatg cctggttgct ttaatttcct ccggaaaaaa
     1261 cttttcttta aaacatcaca ccaccaccac caccactaat ctagcgagca ccaccaccac
     1321 caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgc
//
