LOCUS       sequence-70831-e         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Aranko AS, Oeemig JS, Kajander T, Iwai H
  TITLE     Intermolecular domain swapping induces intein-mediated protein 
            alternative splicing.
  JOURNAL   Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320.
  PUBMED    23974115
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320."
COMMENT     Sequence Label: pSADuet760
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..91
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ataattttgt ttaactttaa taaggagata taccatgggc agcagccatc atcatcatca
       61 tcacagcagc ggcctggtgc cgcgcggcag cctaggacag tacaaactta tcctgaacgg
      121 taaaaccctg aaaggtgaaa ccaccaccga agctgttgac gctgctaccg cggaaaaagt
      181 tttcaaacag tacgctaacg acaacggtgt tgacggtgaa tggacctacg acgacgctac
      241 caaaacctac accgtaacgg aaggatccta tagtgttgat tacaacgaac caataatcat
      301 taaagagaat ggagaaataa aagttgttaa aattggagaa cttatagata aaattattga
      361 aaactcagag aatattagaa gagagggcat cttagagata gcaaaatgta aaggtattga
      421 agttattgcc tttaacagca attacaaatt taaattcatg cctgtttcgg aggtttcaag
      481 gcatccagtt agtgagatgt ttgaaatagt tgttgaaggg aataaaaagg ttagagttac
      541 cagaagccat agtgtcttta ccataagaga taatgaggta gttccaataa gagttgatga
      601 gctaaaagtt ggagatatat tagttttagc aaagaattct gatttcatat tcttaaaaat
      661 taaagagatt aataaggtag agccaaccag tggatatgcc tatgatttaa ctgttccaaa
      721 tgcagaaaac ttcgttgctg gatttggagg atttgtatta cacaacacca tccacggtac
      781 ccagtacaaa cttatcctga acggtaaaac cctgaaaggt gaaaccacca ccgaagctgt
      841 tgacgctgct accgcggaaa aagttttcaa acagtacgct aacgacaacg gtgttgacgg
      901 tgaatggacc tacgacgacg ctaccaaaac ctacaccgta acggaataag cttggctgtt
      961 ttggcggctc gagtc
//
