LOCUS       sequence-7084-ex        1384 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Stennicke HR, Deveraux QL, Humke EW, Reed JC, Dixit VM, Salvesen GS
  TITLE     Caspase-9 can be activated without proteolytic processing.
  JOURNAL   J Biol Chem. 1999 Mar 26. 274(13):8359-62.
  PUBMED    10085063
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1999 Mar 26. 274(13):8359-62."
COMMENT     Sequence Label: pET23b-Casp9 C287A-His
FEATURES             Location/Qualifiers
     source          1..1384
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             complement(778..789)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             1297..1314
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1327..1344
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtttccctct agaaataatt ttgtttaact ttaagaagga gatataccat ggacgaagcg
       61 gatcggcggc tcctgcggcg gtgccggctg cggctggtgg aagagctgca ggtggaccag
      121 ctctgggacg tcctgctgag ccgcgagctg ttcaggcccc atatgatcga ggacatccag
      181 cgggcaggct ctggatctcg gcgggatcag gccaggcagc tgatcataga tctggagact
      241 cgagggagtc aggctcttcc tttgttcatc tcctgcttag aggacacagg ccaggacatg
      301 ctggcttcgt ttctgcgaac taacaggcaa gcagcaaagt tgtcgaagcc aaccctagaa
      361 aaccttaccc cagtggtgct cagaccagag attcgcaaac cagaggttct cagaccggaa
      421 acacccagac cagtggacat tggttctgga ggattcggtg atgtcggtgc tcttgagagt
      481 ttgaggggaa atgcagattt ggcttacatc ctgagcatgg agccctgtgg ccactgcctc
      541 attatcaaca atgtgaactt ctgccgtgag tccgggctcc gcacccgcac tggctccaac
      601 atcgactgtg agaagttgcg gcgtcgcttc tcctcgctgc atttcatggt ggaggtgaag
      661 ggcgacctga ctgccaagaa aatggtgctg gctttgctgg agctggcgcg gcaggaccac
      721 ggtgctctgg actgctgcgt ggtggtcatt ctctctcacg gctgtcaggc cagccacctg
      781 cagttcccag gggctgtcta cggcacagat ggatgccctg tgtcggtcga gaagattgtg
      841 aacatcttca atgggaccag ctgccccagc ctgggaggga agcccaagct ctttttcatc
      901 caggccgcgg gtggggagca gaaagaccat gggtttgagg tggcctccac ttcccctgaa
      961 gacgagtccc ctggcagtaa ccccgagcca gatgccaccc cgttccagga aggtttgagg
     1021 accttcgacc agctggacgc catatctagt ttgcccacac ccagtgacat ctttgtgtcc
     1081 tactctactt tcccaggttt tgtttcctgg agggacccca agagtggctc ctggtacgtt
     1141 gagaccctgg acgacatctt tgagcagtgg gctcactctg aagacctgca gtccctcctg
     1201 cttagggtcg ctaatgctgt ttcggtgaaa gggatttata aacagatgcc tggttgcttt
     1261 aatttcctcc ggaaaaaact tttctttaaa acatcacacc accaccacca ccactaatct
     1321 agcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct
     1381 gagt
//
