LOCUS       sequence-70852-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion 
            protein for periplasmic translocation via PelB signal sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Dammeyer T, Timmis KN, Tinnefeld P
  TITLE     Broad host range vectors for expression of proteins with (Twin-) 
            Strep-tag, His-tag and engineered, export optimized yellow 
            fluorescent protein.
  JOURNAL   Microb Cell Fact. 2013 May 20;12(1):49.
  PUBMED    23687945
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-05-20"; volume: "12(1)"; pages: "49"
COMMENT     Sequence Label: pTDpelB-C_sfYFPTwinStrep
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(83..106,107..142,143..166,167..172))
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
ORIGIN
        1 attactggat ctatcaacag gagtccaaga ctagtcgcca gggttttccc agtcacgacg
       61 cggccgcaag ctttcatcat tatttttcga actgcgggtg gctccacgcc gaacctcccg
      121 atccacctcc ggaacctcca cctttctcga actgcgggtg gctccaagcg ctctcgagtg
      181 cggccgctct agacttgtac agttcgtcca tgcccagggt gatgccggcg gcggtcacaa
      241 attccagcag caccatgtgg tcgcgctttt cgttcgggtc cttcgacagc ttcgactggt
      301 acgacaggta gtggttgtcc ggcagcagca ccgggccgtc gccgatcggg gtgttctgct
      361 ggtagtggtc ggccagctgc acgccgccgt cttccacgtt gtggcggatc ttgaagttgg
      421 ccttgatgcc gttcttctgc ttgtcggcgg tgatgtacac gttgtgcgag ttgaagttgt
      481 attccagctt gtggcccagg atgttgccgt cttccttgaa gtcgatgccc ttcagttcga
      541 tgcggttcac cagggtgtcg ccttcgaact tcacttcggc gcgggtcttg taggtgccgt
      601 cgtccttgaa cgagatggtg cgttcctgca cgtagccttc cggcatggcc gacttgaaga
      661 agtcgtgctg cttcatgtgg tccgggtagc gggcgaagca ctgcaggccg tagcccaggg
      721 tggtcaccag ggtcggccac ggcaccggca gcttgccggt ggtgcagatg aacttcaggg
      781 tcagcttgcc gttggtggcg tcgccttcgc cttcgcccga cacgcggaac ttgtggccgt
      841 tcacgtcgcc gtccagttcc accaggatcg gcaccacgcc ggtgaacagt tcttcgccct
      901 tcgacaccga attcggatcg gccatgg
//
