LOCUS       sequence-70853-e        1719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac backbone, CAG promoter driving murine Tfe3, Hygromycin 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Betschinger J, Nichols J, Dietmann S, Corrin PD, Paddison PJ, Smith 
            A
  TITLE     Exit from pluripotency is gated by intracellular redistribution of 
            the bHLH transcription factor Tfe3.
  JOURNAL   Cell. 2013 Apr 11;153(2):335-47. doi: 10.1016/j.cell.2013.03.012.
  PUBMED    23582324
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.03"; journalName: "Cell"; date: "2013-04-11- 
            11"; volume: "153"; issue: "2"; pages: "335-47"
COMMENT     Sequence Label: pPB-CAG-Tfe3-pgk-hph
FEATURES             Location/Qualifiers
     source          1..1719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtctcatg cagccgagcc agctcgggac gccgtagagg ccagcgcgga gggccctcga
       61 gccgtgttcc tgctattgga agagcgcagg ccagctgagt cggcccagct gctcagcctg
      121 aattctttgc ttccggaatc cgggattgtt gctgatatcg agttagaaaa catcctcgat
      181 cctgacagct tctacgagct caaaagccaa cccctatccc tccgctccag cctcccaata
      241 tcactgcagg ccacaccgac caccccagct acactctctg cgtcgtcttc tgcagggggc
      301 tcccggaccc ctgccatgtc atcttcttca tcacgggtct tgctgcgtca gcagcttatg
      361 agggcccagg ctcaggaaca ggagaggcgt gagcggcggg aacaggcagc ggccgctccc
      421 ttccccagcc ctgcgcccgc ctcaccagcc atctctgtga ttggcgtgtc tgctggtggc
      481 cacacactga gtcgtccacc tcctgctcag gtgcccaggg aggtactcaa ggttcagacc
      541 cacctcgaga accctacacg ctaccacctg cagcaagctc gccggcagca ggtcaaacag
      601 tacttgtcta ccacacttgg gcccaagctg gcttcccagg ctctcacccc accaccaggg
      661 ccttccagtg cccagccact tcctgcccct gaaactgctc atgccaccgg tcctacaggc
      721 agtgctccta acagccccat ggcgctgctc accattgggt ccagctcaga aaaggagatt
      781 gatgatgtca ttgatgagat catcagcctg gagtccagtt acaacgatga gatgctcagc
      841 tatcttccag gaggcactgc agggctgcag ctccccagca cgctgccggt gtctggaaat
      901 ctacttgatg tgtacagcag tcagggagtg gctaccccag ctatcaccgt cagcaattcc
      961 tgtccagctg agctgcctaa catcaaacgc gagatctccg aaaccgaggc aaaggccctt
     1021 ttgaaggagc gacagaagaa agacaatcac aacctaattg aacgacgcag gcgattcaac
     1081 attaacgata ggatcaaaga gctgggcacc ctcatcccca agtccaatga tccggagatg
     1141 cgctggaaca agggcaccat cctgaaggca tctgtggatt acatccgcaa attacagaag
     1201 gaacagcaac gctccaaaga cctggagagc cggcagcggt ccctggaaca agccaaccga
     1261 agcctgcagc tccggattca ggagctagaa ctgcaggccc agatccatgg tctgccagtc
     1321 cctcccaacc caggactgct ctccctaacc acgagttcgg tctctgacag cctcaagcca
     1381 gaacagctgg acattgagga ggagggcagg cctagcacaa cgttccatgt atcgggagga
     1441 cctgcccaga acgctcctcc acaacagcct ccagcaccac cttcggatgc ccttctggac
     1501 ctacactttc ccagcgacca cttgggagac ctgggggacc ccttccacct agggctagag
     1561 gacattctga tggaggagga ggggatggtg ggaggactgt cagggggtgc cctgtcccca
     1621 ctgcgggctg cttctgaccc cctgctttct tcagtatccc cggctgtttc caaggccagc
     1681 agccgccgta gcagcttcag catggaagag gagtcctga
//
