LOCUS       sequence-70855-e        2667 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac backbone, CAG promoter driving murine Tfe3-ERT2 fusion, 
            Hygromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Betschinger J, Nichols J, Dietmann S, Corrin PD, Paddison PJ, Smith 
            A
  TITLE     Exit from pluripotency is gated by intracellular redistribution of 
            the bHLH transcription factor Tfe3.
  JOURNAL   Cell. 2013 Apr 11;153(2):335-47. doi: 10.1016/j.cell.2013.03.012.
  PUBMED    23582324
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.03"; journalName: "Cell"; date: "2013-04-11- 
            11"; volume: "153"; issue: "2"; pages: "335-47"
COMMENT     Sequence Label: pPB-CAG-Tfe3::ERT2-pgk-hph
FEATURES             Location/Qualifiers
     source          1..2667
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1729..2664
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLIPSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             1777..2667
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLIPSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
ORIGIN
        1 atgtctcatg cagccgagcc agctcgggac gccgtagagg ccagcgcgga gggccctcga
       61 gccgtgttcc tgctattgga agagcgcagg ccagctgagt cggcccagct gctcagcctg
      121 aattctttgc ttccggaatc cgggattgtt gctgatatcg agttagaaaa catcctcgat
      181 cctgacagct tctacgagct caaaagccaa cccctatccc tccgctccag cctcccaata
      241 tcactgcagg ccacaccgac caccccagct acactctctg cgtcgtcttc tgcagggggc
      301 tcccggaccc ctgccatgtc atcttcttca tcacgggtct tgctgcgtca gcagcttatg
      361 agggcccagg ctcaggaaca ggagaggcgt gagcggcggg aacaggcagc ggccgctccc
      421 ttccccagcc ctgcgcccgc ctcaccagcc atctctgtga ttggcgtgtc tgctggtggc
      481 cacacactga gtcgtccacc tcctgctcag gtgcccaggg aggtactcaa ggttcagacc
      541 cacctcgaga accctacacg ctaccacctg cagcaagctc gccggcagca ggtcaaacag
      601 tacttgtcta ccacacttgg gcccaagctg gcttcccagg ctctcacccc accaccaggg
      661 ccttccagtg cccagccact tcctgcccct gaaactgctc atgccaccgg tcctacaggc
      721 agtgctccta acagccccat ggcgctgctc accattgggt ccagctcaga aaaggagatt
      781 gatgatgtca ttgatgagat catcagcctg gagtccagtt acaacgatga gatgctcagc
      841 tatcttccag gaggcactgc agggctgcag ctccccagca cgctgccggt gtctggaaat
      901 ctacttgatg tgtacagcag tcagggagtg gctaccccag ctatcaccgt cagcaattcc
      961 tgtccagctg agctgcctaa catcaaacgc gagatctccg aaaccgaggc aaaggccctt
     1021 ttgaaggagc gacagaagaa agacaatcac aacctaattg aacgacgcag gcgattcaac
     1081 attaacgata ggatcaaaga gctgggcacc ctcatcccca agtccaatga tccggagatg
     1141 cgctggaaca agggcaccat cctgaaggca tctgtggatt acatccgcaa attacagaag
     1201 gaacagcaac gctccaaaga cctggagagc cggcagcggt ccctggaaca agccaaccga
     1261 agcctgcagc tccggattca ggagctagaa ctgcaggccc agatccatgg tctgccagtc
     1321 cctcccaacc caggactgct ctccctaacc acgagttcgg tctctgacag cctcaagcca
     1381 gaacagctgg acattgagga ggagggcagg cctagcacaa cgttccatgt atcgggagga
     1441 cctgcccaga acgctcctcc acaacagcct ccagcaccac cttcggatgc ccttctggac
     1501 ctacactttc ccagcgacca cttgggagac ctgggggacc ccttccacct agggctagag
     1561 gacattctga tggaggagga ggggatggtg ggaggactgt cagggggtgc cctgtcccca
     1621 ctgcgggctg cttctgaccc cctgctttct tcagtatccc cggctgtttc caaggccagc
     1681 agccgccgta gcagcttcag catggaagag gagtccctcg agccatctgc tggagacatg
     1741 agagctgcca acctttggcc aagcccgctc atgatcaaac gctctaagaa gaacagcctg
     1801 gccttgtccc tgacggccga ccagatggtc agtgccttgt tggatgctga gccccccata
     1861 ctctattccg agtatgatcc taccagaccc ttcagtgaag cttcgatgat gggcttactg
     1921 accaacctgg cagacaggga gctggttcac atgatcaact gggcgaagag ggtgccaggc
     1981 tttgtggatt tgaccctcca tgatcaggtc caccttctag aatgtgcctg gctagagatc
     2041 ctgatgattg gtctcgtctg gcgctccatg gagcacccag tgaagctact gtttgctcct
     2101 aacttgctct tggacaggaa ccagggaaaa tgtgtagagg gcatggtgga gatcttcgac
     2161 atgctgctgg ctacatcatc tcggttccgc atgatgaatc tgcagggaga ggagtttgtg
     2221 tgcctcaaat ctattatttt gcttaattct ggagtgtaca catttctgtc cagcaccctg
     2281 aagtctctgg aagagaagga ccatatccac cgagtcctgg acaagatcac agacactttg
     2341 atccacctga tggccaaggc aggcctgacc ctgcagcagc agcaccagcg gctggcccag
     2401 ctcctcctca tcccctccca catcaggcac atgagtaaca aaggcatgga gcatctgtac
     2461 agcatgaagt gcaagaacgt ggtgcccctc tatgacctgc tgctggaggc ggcggacgcc
     2521 caccgcctac atgcgcccac tagccgtgga ggggcatccg tggaggagac ggaccaaagc
     2581 cacttggcca ctgcgggctc tacttcatcg cattccttgc aaaagtatta catcacgggg
     2641 gaggcagagg gtttccctgc cacagtc
//
