LOCUS       sequence-70857-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Ma AC, Lee HB, Clark KJ, Ekker SC
  TITLE     High Efficiency In Vivo Genome Engineering with a Simplified 15-RVD 
            GoldyTALEN Design.
  JOURNAL   PLoS One. 2013 May 29;8(5):e65259. doi: 
            10.1371/journal.pone.0065259. Print 2013.
  PUBMED    23734242
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-05-29"; volume: "8(5):e65259. doi"; pages: " 
            10.1371/journal.pone.0065259. Print 2013"
COMMENT     Sequence Label: pFUSB4_CTAA
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..27
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11..27
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcacgacgtt gtaaaacgac ggccagtctt aagcgtctca tggcctgacc ccggaccaag
       61 tggtggctat cgccagccac gatggcggca agcaagcgct cgaaacggtg cagcggctgt
      121 tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct atcgccagca
      181 acggtggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg
      241 accatggcct gaccccggac caagtggtgg ctatcgccag caacattggc ggcaagcaag
      301 cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg
      361 accaagtggt ggctatcgcc agcaacattg gcggcaagca agcgctcgaa acggtgcagc
      421 ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg gtggctatcg
      481 agacgtctag accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg
      541 gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt
      601 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
      661 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg
      721 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
      781 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
      841 cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcaga
//
