LOCUS       sequence-70861-e        1740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac backbone, CAG promoter driving murine 3xFLAG-Flcn fusion, 
            Hygromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Betschinger J, Nichols J, Dietmann S, Corrin PD, Paddison PJ, Smith 
            A
  TITLE     Exit from pluripotency is gated by intracellular redistribution of 
            the bHLH transcription factor Tfe3.
  JOURNAL   Cell. 2013 Apr 11;153(2):335-47. doi: 10.1016/j.cell.2013.03.012.
  PUBMED    23582324
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.03"; journalName: "Cell"; date: "2013-04-11- 
            11"; volume: "153"; issue: "2"; pages: "335-47"
COMMENT     Sequence Label: pPB-CAG-3xFLAG::Flcn-pgk-hph
FEATURES             Location/Qualifiers
     source          1..1740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaacgcca tagtcgccct ctgccacttc tgcgagctcc atggcccccg cacgctcttc
       61 tgcacggaag ttctacacgc tcccctgccc cagggggccg gaagtgggga cagtcctgac
      121 caggttgagc aggctgagga ggaggagggt ggcattcaga tgagcagccg ggtccgtgcc
      181 cacagcccag ccgagggtgc cagcagtgag tccagcagcc cggggcccaa gaagtcggac
      241 atgtgtgagg gctgccggtc acttgccgta gggcacccag gctatatcag tcatgataaa
      301 gagacctcta ttaagtacgt cagtcaccag caccccaacc acccgcagct cttcagcatc
      361 gtccgccagg cctgtgtccg gagcctgagc tgtgaggtat gccctggtcg tgaaggcccc
      421 atcttctttg gtgatgagca gcacggcttt gtgttcagcc acaccttctt catcaaagac
      481 agcctggcca gaggcttcca gcgctggtac agcatcatcg ccatcatgat ggatcggatc
      541 tacctcatca actcctggcc cttcctgctg gggaggatcc gcggcatcat cagtgagctc
      601 caggccaagg ccttcaaggt gtttgaggca gagcagtttg gatgtccaca gcgtgcccag
      661 aggatgaaca ctgccttcac gcccttcctg caccagagga acggcaacgc tgcccgctct
      721 ctgacctcct tgaccagtga tgacaacttg tgggcgtgtc tgcacacttc ctttgcctgg
      781 ctcctgaagg catgcggtag caggctgaca gaaaagctct tagagggcgc tcccacagag
      841 gacaccctgg tccagatgga gaagcttgct gacttggagg aagaatcaga aagttgggac
      901 aattctgagg ctgaggagga ggagaaagct cctgttacac cagagggtgc tgaagggcga
      961 gagctgacca gttgcccaac agagtcatcc tttctctcag cctgtgggag ctggcagccc
     1021 ccaaagctta ccggcttcaa gtctcttcga cacatgagac aggtcttggg tgctccatcc
     1081 ttccgtatgt tggcttggca tgtcctcatg gggaatcagg tgatctggaa aagcagagat
     1141 gtgaacctgg tccattcagc gtttgaagtc ctccggacca tgctgcctgt gggctgtgtc
     1201 cgcatcatcc cttacagcag ccagtatgag gaggcctatc gctgcaactt cctggggctc
     1261 agccctcccg tgcctatccc tgcccatgtt ctggcctcag agttcgtagt tgtcgtggag
     1321 gtccacacgg ccactcgctc aaacctccac cctgctgggt gcgaggatga ccagtccctc
     1381 agcaagtatg agtttgtggt gaccagtggt agccctgtgg ctgcagacag agttgggccc
     1441 actatcctga ataagattga agcagctctg accaaccaga acctgtctgt ggatgtggtg
     1501 gaccaatgtc tcatctgcct caaggaggaa tggatgaaca aagtgaaagt cctgtttaaa
     1561 ttcaccaagg tagacagtcg ccccaaggag gacacacaga agctcctaag cgtcctaggc
     1621 gcatcagagg aggacaacgt caaactgctg aagttctgga tgacgggact gagcaaaacc
     1681 tacaagtccc atctcatgtc caccgtccga agccccacag ctacagagtc acggagctga
//
