LOCUS       sequence-70864-e        3498 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac backbone, CAG promoter driving murine 3xFLAG-Fnip1 fusion, 
            Hygromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Betschinger J, Nichols J, Dietmann S, Corrin PD, Paddison PJ, Smith 
            A
  TITLE     Exit from pluripotency is gated by intracellular redistribution of 
            the bHLH transcription factor Tfe3.
  JOURNAL   Cell. 2013 Apr 11;153(2):335-47. doi: 10.1016/j.cell.2013.03.012.
  PUBMED    23582324
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.03"; journalName: "Cell"; date: "2013-04-11- 
            11"; volume: "153"; issue: "2"; pages: "335-47"
COMMENT     Sequence Label: pPB-CAG-3xFLAG::Fnip1-pgk-hph
FEATURES             Location/Qualifiers
     source          1..3498
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggccccca ccctgttcca gaagctcttc agcaagcgga gcggattagg cgcgccggga
       61 cgagacgccc gcgacccgga ctgcgcgttc agttggccct tgccagaatt tgacccaagt
      121 cagattcgac tgatcgtata tcaagactgt gaaagacgag ggagaaatgt attgtttgac
      181 tccagtgtta agagaaaaaa tgaggacaca tcagtatcga agctctgtaa tgatgctcaa
      241 gtcaaggtct ttgggaagtg ctgccagctg aagccaggag gagatagttc ttcctctttg
      301 gatagctcta tcactttatc ttctgatgga aaagaccagt gtcccaaata ccagggttct
      361 cggtgttctt ctgatgccaa tatgcttgga gagatgatgt ttggctcagt agcaatgagc
      421 tacaaaggat ccaccttaaa aattcatcag atccgctcgc ctccacagct tatgcttagc
      481 aaagttttca ccgcacggac tggcagtagt atctgtggga gtctcaatac tctgcaggac
      541 agtcttgaat tcatcaatca ggacagtaat acattgaaag ctgatagcag cacagttagt
      601 aatgggctgc ttggaaatat aggtctttca cagttctgca gccccaggcg ggctttctct
      661 gagcagggtc cgctccgcct gatcaggagt gcctctttct ttgcagttca cagcaaccca
      721 atggacatgc caggaagaga actgaatgag gacagagaca gcggcatagc acgttctgca
      781 tctctgagca gcttgttcat caccccattc ccctccccaa actcttcact tacccgaagc
      841 tgtgctagca gctaccagcg acgttggcga cgtagccaaa caacaagttt ggaaaatggg
      901 gtgtttccta gatggtctgt agaagaaagc tttaatctgt cagatgaaag ttgtggccct
      961 aatccaggaa ttgtgaggaa aaagaagatt gcaattgggg taatcttttc attatccaaa
     1021 gatgaagatg aaaataacaa attcaatgaa ttcttttttt cacattttcc tctctttgaa
     1081 agccacatga acaaattaaa aagtgcaata gaacaggcaa tgaaaatgag ccggagatca
     1141 gctgatgcca gccagagaag tttggcatac aatcgaatac ttgatgccct gactgagttc
     1201 agaacaacaa tttgtaatct ttatacaatg ccaagaattg gggaacctgt ctggcttaca
     1261 atgatgtcag ggactccaga gaagaaccag ctttgccatc gtttcatgaa ggaattcacc
     1321 tttctaatgg aaaatgcctc caaaaatcaa ttcttgccag ctctcattac tgcagttttg
     1381 accaatcatc ttgcctgggt tccaacagtc atgccaaatg gacagccacc tataaaaata
     1441 tttttagaaa aacattcctc tcagagtgtg ggcatgttgg caaagactca tccatataac
     1501 cctctttggg cacaacttgg agatttgtat ggcgctattg gatctcctgt acggttagca
     1561 agaactgtag tcgttggcaa acgacaagac ctggtccaga gactgcttta ttttcttact
     1621 tacttcatca gatgctctga acttcaagaa acccatcttt tagaaaatgg agaagatgaa
     1681 gccattgtta tgccaggcac agtgattact accactttag aaaaggggga aatagaggaa
     1741 tcagagtatg tgctaatcac aatgcataga aacaaaagca gcttgctctt taaagagtca
     1801 gaagagacaa gaactcccaa ctgtaactgt aaatattgca gtcatccagt ccttgggcaa
     1861 aatacagaga atgtgtcaca accagaaaga gaagatactc aagacaactc taaggaattg
     1921 ctaggaattt cagatgaatg ccaaaagatt tctcctcctg actgccaaga agaaaatgct
     1981 gttgatgttc aacagtacag agataaatta agaacttgcc ttgacaccaa gttagagaca
     2041 gttgtttgta caggatctgc tccagccgac aaatgtgtgt tgtcagagac atgcttggaa
     2101 ccaagagaag aatcctggca gaataaggaa ttgctggatt cagataatca cacaggcaca
     2161 gcaatgagac ccacaggaat agttgttgaa aagaaacctc cagataagaa tgtgcccagt
     2221 gcattttcct gtgaggtaac tcagacaaag gttacgttct tgattggaga ttctatgtca
     2281 cctgattcag atactgaact tcggagtcag gctgtggtgg atcagattaa cagacatcac
     2341 agcgaaccac taaaggaaga cagaggagtg gctgataagc atcaagaaag taaaataact
     2401 aaggaccaat ctgaagactc tgacacacag aacatagttt ctggagagtc ctgtgagctt
     2461 ccttgttgga gtcattcaga tccagaaagc atgagcttgt ttgatgaata ttttaatgat
     2521 gattcaattg aaaccaggac tattgatgat gttccagtta aaacaagtac agatagtaaa
     2581 gagtattgct gtatgttgga gtatccaaaa agattgtata caaaaactaa caaacagaaa
     2641 agtgaacttt gtaaatgtat agaaacagtt catcaagatt catgtaatgc ctgctttcct
     2701 cagcaggacc agaggaattc cctctccatt cttgtccccc atggggataa agagagttca
     2761 gacaaaaaaa atgctgtagg aactgaatgg gacattccaa gaaatgaaag ttcagatagt
     2821 gcccttggag atagtgagag tgaagacaca ggcccggaca taaggagaca agctggcggt
     2881 tactgtggag gggaccagga agactggaca gaagaggatg agataccttt tcctgggtca
     2941 aaattgattg aagtgagtgc tgttcagccc aacattgcca actttggaag atctttgctg
     3001 ggtggctact gctcatctta tgttcctgac tttgttcttc aaggaattgg aaatgatgag
     3061 aggctccgtc agtgcctggt atcagatttg tctcatgctg tgcagcatcc agtgttagat
     3121 gaaccaatag cagaagctgt ctgtattata gctgatatgg ataaatggac tgttcaagtg
     3181 gccagcagtc agagacgagt aacagataat aaattgggaa aggaggtgtt ggtctctagt
     3241 cttgtttcca acctgcttca ttccactctt cagctttata aacataattt atctccaaac
     3301 ttttgtgtaa tgcaccttga agaccggtta caggagttat acttcaagag caaaatgctg
     3361 tctgagtacc tgaggggaca gatgcgtgtt catgtcaagg agctaggagt ggttcttggg
     3421 attgaatcca gtgaccttcc tcttctggct gcggtagcaa gcacccactc tccatacgtt
     3481 gctcagatac tcctttaa
//
