LOCUS       sequence-70883-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  use to create N-terminal Strep-tagII fusion proteins or dual tagged 
            fusion with N-terminal Strep-tagII and C-terminal 6x His-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Dammeyer T, Timmis KN, Tinnefeld P
  TITLE     Broad host range vectors for expression of proteins with (Twin-) 
            Strep-tag, His-tag and engineered, export optimized yellow 
            fluorescent protein.
  JOURNAL   Microb Cell Fact. 2013 May 20;12(1):49.
  PUBMED    23687945
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-05-20"; volume: "12(1)"; pages: "49"
COMMENT     Sequence Label: pTD-NStrepHis
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(342..347,348..364,365..371)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    379..395
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             455..478
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             575..592
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      650..744
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
       61 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      121 attaatgtaa gttagcgcga attgatctgg tttgacagct tatcatcgac tgcacggtgc
      181 accaatgctt ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa
      241 atcactgcat aattcgtgtc gctcaaggcg cactcccgtt ctggataatg ttttttgcgc
      301 cgacatcata acggttctgg caaatattct gaaatgagct gttgacaatt aatcatccgg
      361 ctcgtataat gtgtggaatt gtgagcggat aacaatttca caccctaggc cgcggccgcg
      421 cgaattgttt aaaggagata tacaaatggc tagctggagc cacccgcagt tcgaaaaagg
      481 cgccgagacc gcggtcccga attcgagctc ggtacccggg gatccctcga ggtcgacctg
      541 cagggggacc atggtctcag gcctgagagg atcgcatcac catcaccatc actaatttaa
      601 aaagcttgcg gccgcgtcgt gactgggaaa accctggcga ctagtcttgg actcctgttg
      661 atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc
      721 cgggcgtttt ttattggtga gaatccaggg gtccccaata attacgattt acgtatttaa
      781 atgaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
      841 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
      901 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
//
