LOCUS       sequence-70889-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Ma AC, Lee HB, Clark KJ, Ekker SC
  TITLE     High Efficiency In Vivo Genome Engineering with a Simplified 15-RVD 
            GoldyTALEN Design.
  JOURNAL   PLoS One. 2013 May 29;8(5):e65259. doi: 
            10.1371/journal.pone.0065259. Print 2013.
  PUBMED    23734242
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-05-29"; volume: "8(5):e65259. doi"; pages: " 
            10.1371/journal.pone.0065259. Print 2013"
COMMENT     Sequence Label: pFUSB4_CTGG
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..31
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15..31
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccagtcacga cgttgtaaaa cgacggccag tcttaagcgt ctcatggcct gaccccggac
       61 caagtggtgg ctatcgccag ccacgatggc ggcaagcaag cgctcgaaac ggtgcagcgg
      121 ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc
      181 agcaacggtg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc
      241 caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacaa tggcggcaag
      301 caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc
      361 ccggaccaag tggtggctat cgccagcaac aatggcggca agcaagcgct cgaaacggtg
      421 cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct
      481 atcgagacgt ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg
      541 ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt
      601 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
      661 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
      721 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
      781 gtttgatgtt atggagcagc aacgatgtta cgcagca
//
