LOCUS       sequence-70895-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  use to create C-terminal Twin-Strep-tag fusion proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Dammeyer T, Timmis KN, Tinnefeld P
  TITLE     Broad host range vectors for expression of proteins with (Twin-) 
            Strep-tag, His-tag and engineered, export optimized yellow 
            fluorescent protein.
  JOURNAL   Microb Cell Fact. 2013 May 20;12(1):49.
  PUBMED    23687945
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-05-20"; volume: "12(1)"; pages: "49"
COMMENT     Sequence Label: pTD-CTwinStrep
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(357..362,363..379,380..386)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    394..410
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             522..545
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             582..605
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     terminator      663..757
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc
       61 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
      121 agtgagcgca acgcaattaa tgtaagttag cgcgaattga tctggtttga cagcttatca
      181 tcgactgcac ggtgcaccaa tgcttctggc gtcaggcagc catcggaagc tgtggtatgg
      241 ctgtgcaggt cgtaaatcac tgcataattc gtgtcgctca aggcgcactc ccgttctgga
      301 taatgttttt tgcgccgaca tcataacggt tctggcaaat attctgaaat gagctgttga
      361 caattaatca tccggctcgt ataatgtgtg gaattgtgag cggataacaa tttcacaccc
      421 taggccgcgg ccgcgcgaat tgttaacttt aagaaggaga tatacatatg gatccgaatt
      481 cgagctccgt cgactctaga gcggccgcac tcgagagcgc ttggagccac ccgcagttcg
      541 agaaaggtgg aggttccgga ggtggatcgg gaggtggatc gtggagccac ccgcagttcg
      601 aaaaataatg atgaaagctt gcggccgcgt cgtgactggg aaaaccctgg cgactagtct
      661 tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac
      721 gctcggttgc cgccgggcgt tttttattgg tgagaatcca ggggtcccca ataattacga
      781 tttacgtatt taaatgaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
      841 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
      901 aagcgcgtta cgccgtgggt cgatgtttga tgttatgg
//
