LOCUS       sequence-70915-e         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Ma AC, Lee HB, Clark KJ, Ekker SC
  TITLE     High Efficiency In Vivo Genome Engineering with a Simplified 15-RVD 
            GoldyTALEN Design.
  JOURNAL   PLoS One. 2013 May 29;8(5):e65259. doi: 
            10.1371/journal.pone.0065259. Print 2013.
  PUBMED    23734242
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-05-29"; volume: "8(5):e65259. doi"; pages: " 
            10.1371/journal.pone.0065259. Print 2013"
COMMENT     Sequence Label: pFUSB4_CTCA
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..34
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     18..34
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tttccagtca cgacgttgta aaacgacggc cagtcttaag cgtctcatgg cctgaccccg
       61 gaccaagtgg tggctatcgc cagccacgat ggcggcaagc aagcgctcga aacggtgcag
      121 cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc
      181 gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg
      241 tgccaggacc atggcctgac tccggaccaa gtggtggcta tcgccagcca cgatggcggc
      301 aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg
      361 accccggacc aagtggtggc tatcgccagc aacattggcg gcaagcaagc gctcgaaacg
      421 gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg
      481 gctatcgaga cgtctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg
      541 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct
      601 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
      661 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
      721 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
      781 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
      841 agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
      901 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctcc
//
