LOCUS       sequence-70935-e         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To evaluate the efficacy of silencing and the extent of 
            cooperativity directed by a small silencing RNA bound at one or 
            multiple sites on an mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Broderick JA, Salomon WE, Ryder SP, Aronin N, Zamore PD
  TITLE     Argonaute protein identity and pairing geometry determine 
            cooperativity in mammalian RNA silencing.
  JOURNAL   RNA. 2011 Oct;17(10):1858-69. Epub 2011 Aug 30.
  PUBMED    21878547
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "2011-10"; volume: "17(10)"; pages: "1858-69. Epub 2011 Aug 
            30"
COMMENT     Sequence Label: mutpRL-TK 1xUTR
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(44..979)
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
ORIGIN
        1 cggccgctct agggcccata gctataatga aatgcctcta gaattattgt tcatttttga
       61 gaactcgctc aacgaacgat ttgatatatt ttcccatttc atcaggtgca tcttcttgcg
      121 aaaaatgaag accttttact ttgacaaatt cagtattagg aaacttcttg gcaccttcaa
      181 caatagcatt ggaaaagaat cctgggtccg attcaataaa catttttggt aaatcatcac
      241 ttgcacgtag ataagcatta taattcctaa caatttgtac aacgtcaggt ttaccacctt
      301 ttactaacgg gatttcacga ggccatgata atgttggacg acgaacttca cctttctctt
      361 tgaatggttc aagatatgct gcaaattctt ctggttctaa ctttctcatg atttttgatg
      421 gcaacatggt ttccacgaag aagttattct ccaaaaccat tttttctcct tcttcagatt
      481 tgatcaacgc aatatcttct tcaatatcag gccattcatc ccatgattca atcacatcta
      541 ctacactttc agcgtgaact attgctttga tcttatcttg atgctcatag gagtaatgaa
      601 atgccaaaca agcaccccaa tcatggccga caaaaatgat cttctttggt aaattaagaa
      661 gttcaaacca tgcagtaaga tatttgtaat gatcaagtaa cctataagaa ccattaccag
      721 atttgcctga tttgcccata ccaataaggt ctggtataat acaccgcgct actggctcaa
      781 tatgtggcac aacatgtcgc cataaataag aagaggccgc gttaccatgt aaaaaaataa
      841 cagcattttc tgcatgtttt tctgaatcat aataattaat aaatgaatca agaacattca
      901 tttgtttaca tctggcccac cactgcggac cagttatcat ccgtttcctt tgttctggat
      961 cataaacttt cgaagtcatg
//
