LOCUS       sequence-70938-e         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To evaluate the efficacy of silencing and the extent of 
            cooperativity directed by a small silencing RNA bound at one or 
            multiple sites on an mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Broderick JA, Salomon WE, Ryder SP, Aronin N, Zamore PD
  TITLE     Argonaute protein identity and pairing geometry determine 
            cooperativity in mammalian RNA silencing.
  JOURNAL   RNA. 2011 Oct;17(10):1858-69. Epub 2011 Aug 30.
  PUBMED    21878547
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "2011-10"; volume: "17(10)"; pages: "1858-69. Epub 2011 Aug 
            30"
COMMENT     Sequence Label: mutpRL-TK 3xUTR
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(47..982)
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
ORIGIN
        1 gagcggccgc tctagggccc atagctataa tgaaatgcct ctagaattat tgttcatttt
       61 tgagaactcg ctcaacgaac gatttgatat attttcccat ttcatcaggt gcatcttctt
      121 gcgaaaaatg aagacctttt actttgacaa attcagtatt aggaaacttc ttggcacctt
      181 caacaatagc attggaaaag aatcctgggt ccgattcaat aaacattttt ggtaaatcat
      241 cacttgcacg tagataagca ttataattcc taacaatttg tacaacgtca ggtttaccac
      301 cttttactaa cgggatttca cgaggccatg ataatgttgg acgacgaact tcacctttct
      361 ctttgaatgg ttcaagatat gctgcaaatt cttctggttc taactttctc atgatttttg
      421 atggcaacat ggtttccacg aagaagttat tctccaaaac cattttttct ccttcttcag
      481 atttgatcaa cgcaatatct tcttcaatat caggccattc atcccatgat tcaatcacat
      541 ctactacact ttcagcgtga actattgctt tgatcttatc ttgatgctca taggagtaat
      601 gaaatgccaa acaagcaccc caatcatggc cgacaaaaat gatcttcttt ggtaaattaa
      661 gaagttcaaa ccatgcagta agatatttgt aatgatcaag taacctataa gaaccattac
      721 cagatttgcc tgatttgccc ataccaataa ggtctggtat aatacaccgc gctactggct
      781 caatatgtgg cacaacatgt cgccataaat aagaagaggc cgcgttacca tgtaaaaaaa
      841 taacagcatt ttctgcatgt ttttctgaat cataataatt aataaatgaa tcaagaacat
      901 tcatttgttt acatctggcc caccactgcg gaccagttat catccgtttc ctttgttctg
      961 gatcataaac tttcgaagtc at
//
