LOCUS       sequence-70953-e        1135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S
  TITLE     A modular cloning system for standardized assembly of multigene 
            constructs.
  JOURNAL   PLoS One. 2011 Feb 18;6(2):e16765. doi: 
            10.1371/journal.pone.0016765.
  PUBMED    21364738
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-02-18"; volume: "6(2):e16765. doi"; pages: " 
            10.1371/journal.pone.0016765"
COMMENT     Sequence Label: pICH41722
FEATURES             Location/Qualifiers
     source          1..1135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(555..572)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(555..571)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(564..586)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(780..799)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     899..921
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(959..977)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaggat ccccttgaag
       61 acaagcaaga ggatgcacat gtgaccgagg gattgtcttc gtggaaactt gggacttcag
      121 attccacccc ttgcaagcgt ggaaagcctt ccgaccaagg gaagtgtcgg atgtagagga
      181 aatggaaagt ttgttctcag atggggactt gcttgattgc ttcacaagaa tgccagctta
      241 tgcggtaaac gcagaggaag atttagctgc aatcaggaaa acgcccgaga tggatgtcgg
      301 tcaagaagtt aaagaacctg caggagacag aaatcaatac tcaaaccctg cagaaacttt
      361 cctcaacaag ctccacagga aacacagtag ggaggtgaaa caccaggccg caaagaaagc
      421 taaacgccta gctgaaatcc aggagtcaat gagagctgaa ggtgatgccg aaccaaatga
      481 aataagcggg ggcatggggg caatacccag caacgccgaa cttcctgggc ccaaagggta
      541 ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
      601 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
      661 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
      721 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
      781 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
      841 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccatctcc
      901 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag tcgaaagggc
      961 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     1021 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     1081 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atatt
//
