LOCUS       sequence-70954-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Ma AC, Lee HB, Clark KJ, Ekker SC
  TITLE     High Efficiency In Vivo Genome Engineering with a Simplified 15-RVD 
            GoldyTALEN Design.
  JOURNAL   PLoS One. 2013 May 29;8(5):e65259. doi: 
            10.1371/journal.pone.0065259. Print 2013.
  PUBMED    23734242
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-05-29"; volume: "8(5):e65259. doi"; pages: " 
            10.1371/journal.pone.0065259. Print 2013"
COMMENT     Sequence Label: pFUSB4_GACG
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..23
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7..23
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacgttgtaa aacgacggcc agtcttaagc gtctcatggc ctgaccccgg accaagtggt
       61 ggctatcgcc agcaacaatg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc
      121 ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacat
      181 tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca
      241 tggcctgact ccggaccaag tggtggctat cgccagccac gatggcggca agcaagcgct
      301 cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca
      361 agtggtggct atcgccagca acaatggcgg caagcaagcg ctcgaaacgg tgcagcggct
      421 gttgccggtg ctgtgccagg accatggcct gaccccggac caagtggtgg ctatcgagac
      481 gtctagacca gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga
      541 cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt
      601 aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
      661 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta
      721 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
      781 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt
      841 atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
      901 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt a
//
