LOCUS       sequence-70960-e        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S
  TITLE     A modular cloning system for standardized assembly of multigene 
            constructs.
  JOURNAL   PLoS One. 2011 Feb 18;6(2):e16765. doi: 
            10.1371/journal.pone.0016765.
  PUBMED    21364738
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-02-18"; volume: "6(2):e16765. doi"; pages: " 
            10.1371/journal.pone.0016765"
COMMENT     Sequence Label: pICH50914
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(563..580)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(563..579)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(572..594)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(788..807)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     907..929
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(967..985)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaggat cccctttgaa
       61 gacaatgtgt gagaccgagg atgcacatgt gaccgaggga ttgtcttcgt ggaaacttgg
      121 gacttcagat tccacccctt gcaagcgtgg aaagccttcc gaccaaggga agtgtcggat
      181 gtagaggaaa tggaaagttt gttctcagat ggggacttgc ttgattgctt cacaagaatg
      241 ccagcttatg cggtaaacgc agaggaagat ttagctgcaa tcaggaaaac gcccgagatg
      301 gatgtcggtc aagaagttaa agaacctgca ggagacagaa atcaatactc aaaccctgca
      361 gaaactttcc tcaacaagct ccacaggaaa cacagtaggg aggtgaaaca ccaggccgca
      421 aagaaagcta aacgcctagc tgaaatccag gagtcaatga gagctgaagg tgatgccgaa
      481 ccaaatgaaa taagcggggg catgggggca atacccagca acgccgaact tcctgggccc
      541 aaagggtacc gagctcgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
      601 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
      661 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
      721 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
      781 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
      841 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
      901 ccatctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagtc
      961 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     1021 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1081 aaatacattc
//
