LOCUS       sequence-70971-e        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S
  TITLE     A modular cloning system for standardized assembly of multigene 
            constructs.
  JOURNAL   PLoS One. 2011 Feb 18;6(2):e16765. doi: 
            10.1371/journal.pone.0016765.
  PUBMED    21364738
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-02-18"; volume: "6(2):e16765. doi"; pages: " 
            10.1371/journal.pone.0016765"
COMMENT     Sequence Label: pICH79311
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(562..579)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(562..578)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(571..593)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(787..806)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     906..928
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(966..984)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaggat cccctttggt
       61 ctcatgcctt gtcttcgagg atgcacatgt gaccgaggga tgagaccgtg gaaacttggg
      121 acttcagatt ccaccccttg caagcgtgga aagccttccg accaagggaa gtgtcggatg
      181 tagaggaaat ggaaagtttg ttctcagatg gggacttgct tgattgcttc acaagaatgc
      241 cagcttatgc ggtaaacgca gaggaagatt tagctgcaat caggaaaacg cccgagatgg
      301 atgtcggtca agaagttaaa gaacctgcag gagacagaaa tcaatactca aaccctgcag
      361 aaactttcct caacaagctc cacaggaaac acagtaggga ggtgaaacac caggccgcaa
      421 agaaagctaa acgcctagct gaaatccagg agtcaatgag agctgaaggt gatgccgaac
      481 caaatgaaat aagcgggggc atgggggcaa tacccagcaa cgccgaactt cctgggccca
      541 aagggtaccg agctcgaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
      601 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      661 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
      721 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
      781 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
      841 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
      901 catctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagtcg
      961 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     1021 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaac
//
