LOCUS       sequence-70975-e        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S
  TITLE     A modular cloning system for standardized assembly of multigene 
            constructs.
  JOURNAL   PLoS One. 2011 Feb 18;6(2):e16765. doi: 
            10.1371/journal.pone.0016765.
  PUBMED    21364738
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-02-18"; volume: "6(2):e16765. doi"; pages: " 
            10.1371/journal.pone.0016765"
COMMENT     Sequence Label: pICH89921
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..73
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(74..91)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(74..90)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(83..105)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(299..318)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    456..480
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac
       61 cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
      121 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
      181 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
      241 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
      301 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
      361 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg acgaagacaa
      421 gggacacgaa gtgatccgtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
      481 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta
      541 tccgttcgtc catttgtatg tgccagccgc aagggtgact tcgcctatat tggacgacgg
      601 cgcgcagagg gcgacctctt tttgggttac gattgtagga ttatcactaa aacaatacat
      661 gaacatattc aaatggcaat ctctctaagg cattggaaat aaatacaaat aacagttggg
      721 tggagttttt cgacctgagg gcgttaacct tctgttaacc taaaagctct tgcccaaaca
      781 gcagaatcgg cgctaattgc cagcggcgga acttttccag tttcgcgaaa aatatcgcca
      841 ctggcaagga atgggtttga gatggcgaag tctgtcctaa aagcagcgcc tgtagttgta
      901 gggttgacgg ccttgatgga gcgtcatgcc gatgccctct cgagccaact tcaagcacat
      961 catcttaagg ttttcccgcc gcattccgag aagggcattc gaacattcgg gccatcggag
     1021 gcgtccaagc tgctcggcgt tggcgagtca tatttacggc a
//
