LOCUS       sequence-70978-e        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Renilla luciferase reporter containing siRNA target sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Broderick JA, Salomon WE, Ryder SP, Aronin N, Zamore PD
  TITLE     Argonaute protein identity and pairing geometry determine 
            cooperativity in mammalian RNA silencing.
  JOURNAL   RNA. 2011 Oct;17(10):1858-69. Epub 2011 Aug 30.
  PUBMED    21878547
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "2011-10"; volume: "17(10)"; pages: "1858-69. Epub 2011 Aug 
            30"
COMMENT     Sequence Label: psiCheck2 3xUTR
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             56..991
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
ORIGIN
        1 cattacagct cttaaggcta gagtacttaa tacgactcac tataggctag ccaccatgac
       61 ttcgaaagtt tatgatccag aacaaaggaa acggatgata actggtccgc agtggtgggc
      121 cagatgtaaa caaatgaatg ttcttgattc atttattaat tattatgatt cagaaaaaca
      181 tgcagaaaat gctgttattt ttttacatgg taacgcggcc tcttcttatt tatggcgaca
      241 tgttgtgcca catattgagc cagtagcgcg gtgtattata ccagacctta ttggtatggg
      301 caaatcaggc aaatctggta atggttctta taggttactt gatcattaca aatatcttac
      361 tgcatggttt gaacttctta atttaccaaa gaagatcatt tttgtcggcc atgattgggg
      421 tgcttgtttg gcatttcatt actcctatga gcatcaagat aagatcaaag caatagttca
      481 cgctgaaagt gtagtagatg tgattgaatc atgggatgaa tggcctgata ttgaagaaga
      541 tattgcgttg atcaaatctg aagaaggaga aaaaatggtt ttggagaata acttcttcgt
      601 ggaaaccatg ttgccatcaa aaatcatgag aaagttagaa ccagaagaat ttgcagcata
      661 tcttgaacca ttcaaagaga aaggtgaagt tcgtcgtcca acattatcat ggcctcgtga
      721 aatcccgtta gtaaaaggtg gtaaacctga cgttgtacaa attgttagga attataatgc
      781 ttatctacgt gcaagtgatg atttaccaaa aatgtttatt gaatcggacc caggattctt
      841 ttccaatgct attgttgaag gtgccaagaa gtttcctaat actgaatttg tcaaagtaaa
      901 aggtcttcat ttttcgcaag aagatgcacc tgatgaaatg ggaaaatata tcaaatcgtt
      961 cgttgagcga gttctcaaaa atgaacaata attctagagg catttcatta tagctatggc
//
