LOCUS       sequence-70984-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  contains modified circularly permutated EGFP (mcpGFP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   De Michele R, Ast C, Loque D, Ho CH, Andrade SL, Lanquar V, 
            Grossmann G, Gehne S, Kumke MU, Frommer WB
  TITLE     Fluorescent sensors reporting the activity of ammonium transceptors 
            in live cells.
  JOURNAL   eLife. 2013 Jul 2;2:e00800.
  PUBMED    23840931
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2013-07-2"; volume: "2"; pages: "e00800"
COMMENT     Sequence Label: pGEM-mcpGFP
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    42..66
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             93..344
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNH
                     YLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     281..302
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(408..429)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(669..688)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgctccggc cgccatggcg gccgcgggaa ttcgattggg gacaagtttg tacaaaaaag
       61 caggcttaat cgataacgtc tatatcaagg ccgacaagca gaagaacggc atcaaggcga
      121 acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgcctac cactaccagc
      181 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcgtcc
      241 agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
      301 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggtggt accggtggat
      361 ctatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      421 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      481 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      541 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
      601 agcagcacga cttcttcaag tccgccatgc ccgaaggcta catccaggag cgcaccatct
      661 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      721 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
      781 acaagctgga gtacaaccct aggaacccag ctttctttta caaagggtca atcactagtg
      841 aattcgcggc cgcctgc
//
