LOCUS       sequence-70985-e        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses MepTrac-H194E in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   De Michele R, Ast C, Loque D, Ho CH, Andrade SL, Lanquar V, 
            Grossmann G, Gehne S, Kumke MU, Frommer WB
  TITLE     Fluorescent sensors reporting the activity of ammonium transceptors 
            in live cells.
  JOURNAL   eLife. 2013 Jul 2;2:e00800.
  PUBMED    23840931
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2013-07-2"; volume: "2"; pages: "e00800"
COMMENT     Sequence Label: pDR-meptrac-H194E
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..287)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNH
                     YLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(78..99)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(965..989)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcgcccttgc tcaccataga tccaccggta ccacccttgt acagctcgtc catgccgaga
       61 gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg
      121 tctttgctca gcttggactg gacgctcagg tagtggttgt cgggcagcag cacggggccg
      181 tcgccgatgg gggtgttctg ctggtagtgg taggcgagct gcacgccgcc gtcctcgatg
      241 ttgtggcgga tcttgaagtt cgccttgatg ccgttcttct gcttgtcggc cttgatatag
      301 acgttagaca aacgtgtaac agggtcatta cgcttaccca gtatcaaagc gtaaactaga
      361 ccaccatgtc cagatgttaa ctcgacacat aaaccacctg catagtccaa gctacccaat
      421 ttgaccaacc aaccctcggc attccagacc cagcatgcaa taggacaata aacaatagtc
      481 atccataaaa acaagaacac catcatagga aacaaccttg ccctctcgca ggcaccacct
      541 agcattaggg caccggtgac tgcggcaaac ataccttggt aaacggcaaa cagtatatcg
      601 ggaagagaac tgacactaga tggggctcct aaaacgttac gaaacccaaa gaattccaag
      661 gtaccaataa aaccgttacc tctagtgttg tgtgagaaag ctaatgagta tccccagaaa
      721 aaccattgga aaatacacac ggcggaagcc atcatcgagg cccaaagcaa ggataaagca
      781 tgctttttcc tggataaacc agaatataat aaaccgatac ctgggaccat aatccacaca
      841 cctgctgaag ccacaccgat ccatcccatg ttggccaagt caaattgtgt attcaaatct
      901 gttgtcaacg agttaccacc cgttccttcg cctgtaggcg tacctgtaaa attgtaagac
      961 attaagcctg cttttttgta caaacttgtg atggccgcga attca
//
