LOCUS       sequence-70986-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses AmTrac1;2 in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   De Michele R, Ast C, Loque D, Ho CH, Andrade SL, Lanquar V, 
            Grossmann G, Gehne S, Kumke MU, Frommer WB
  TITLE     Fluorescent sensors reporting the activity of ammonium transceptors 
            in live cells.
  JOURNAL   eLife. 2013 Jul 2;2:e00800.
  PUBMED    23840931
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2013-07-2"; volume: "2"; pages: "e00800"
COMMENT     Sequence Label: pDR-AmTrac1;2
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..292)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNH
                     YLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(83..104)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctcctcgcc cttgctcacc atagatccac cggtaccacc cttgtacagc tcgtccatgc
       61 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
      121 tggggtcttt gctcagcttg gactggacgc tcaggtagtg gttgtcgggc agcagcacgg
      181 ggccgtcgcc gatgggggtg ttctgctggt agtggtaggc gagctgcacg ccgccgtcct
      241 cgatgttgtg gcggatcttg aagttcgcct tgatgccgtt cttctgcttg tcggccttga
      301 tatagacgtt ggacaaccgg tcaaatctac ctattcttgg tccttcaact aacgctccac
      361 ataaaccggc aattccacct accatgtgaa caactcctga acctgcgaaa tcaatagcac
      421 ctgagccaaa caagagattg ttgtcagacc gggacgcgct agcccatcca tcacttgacc
      481 agaaccagtg cgagactgtc gggtaaacaa aaccggtcaa gaaagtagag tagataaggt
      541 aagcaacgaa ttgcgttcgc tcggcgatgg aaccgctagt gattccggcc gcggctatgg
      601 caaaagccca ttggtagagg aaaaagctga agtcggagcc ggggcgttca gggtaagagc
      661 ttaaagcgaa gaagctatgg tggcgaccga tgaatccgtt ggaaggtgta ccaaaggcga
      721 atgcgaatcc gaagaggtag taagagatgg ctccagcggc agcatcaagg acattggtaa
      781 gcatgatgtt catagtgttc ttggctcgga ctgatccagc acaaagcata gcgaaaccga
      841 gctgcatggc aaagacaagg taggcggaga agaggagata cgt
//
