LOCUS       sequence-70987-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses AmTrac-IS in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   De Michele R, Ast C, Loque D, Ho CH, Andrade SL, Lanquar V, 
            Grossmann G, Gehne S, Kumke MU, Frommer WB
  TITLE     Fluorescent sensors reporting the activity of ammonium transceptors 
            in live cells.
  JOURNAL   eLife. 2013 Jul 2;2:e00800.
  PUBMED    23840931
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2013-07-2"; volume: "2"; pages: "e00800"
COMMENT     Sequence Label: pDR-AmTrac-IS
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..287)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNH
                     YLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(78..99)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcgcccttgc tcaccataga tccaccggta ccacccttgt acagctcgtc catgccgaga
       61 gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg
      121 tctttgctca gcttggactg gacgctcagg tagtggttgt cgggcagcag cacggggccg
      181 tcgccgatgg gggtgttctg ctggtagtgg taggcgagct gcacgccgcc gtcctcgatg
      241 ttgtggcgga tcttgaagtt cgccttgatg ccgttcttct gcttgtcggc cttgatatag
      301 acgttagaga ttttctcgaa ccgaccacga cgaggacctt caataagagc accccataaa
      361 cctgctatgc caccaaccat gtgaacaaca ccggagccag caaagtcaat ggctccggtg
      421 ctaaacaaac gatcatccgc tgaacgaaag ggactggccc atccatccgg ggaccaaaac
      481 cagtgagaga caaccgggta aacaaatccg gttaagaaag aagagtatat caagtaagcc
      541 acgaactgag tcctctctgc gatcgaacca cttgtgattc cagcggccgc gattgcgaac
      601 gcccattggt agaggaagaa agagtaatca gctgtgggag tcggaaagtc tctaagagca
      661 aagttgtgtc ttccaatgaa cccttcggag gatcctccaa aggcaaaggc gtaaccaaag
      721 agatagtaga agagtcctcc ggctgcagcg tcaaggacat tggtaagcat gatgttcatc
      781 gtattcttgg ctctaacaga accagcacaa agcatagcga agccgagctg catggcga
//
