LOCUS       sequence-70992-e        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S
  TITLE     A modular cloning system for standardized assembly of multigene 
            constructs.
  JOURNAL   PLoS One. 2011 Feb 18;6(2):e16765. doi: 
            10.1371/journal.pone.0016765.
  PUBMED    21364738
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-02-18"; volume: "6(2):e16765. doi"; pages: " 
            10.1371/journal.pone.0016765"
COMMENT     Sequence Label: pICH82113
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..73
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(74..91)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(74..90)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(83..105)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(299..318)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    455..479
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac
       61 cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
      121 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
      181 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
      241 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
      301 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
      361 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg acggtctcag
      421 ggacacgaag tgatccgttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
      481 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
      541 ccgttcgtcc atttgtatgt gccagccgca agggtgactt cgcctatatt ggacgacggc
      601 gcgcagaggg cgacctcttt ttgggttacg attgtaggat tatcactaaa acaatacatg
      661 aacatattca aatggcaatc tctctaaggc attggaaata aatacaaata acagttgggt
      721 ggagtttttc gacctgaggg cgttaacctt ctgttaacct aaaagctctt gcccaaacag
      781 cagaatcggc gctaattgcc agcggcggaa cttttccagt ttcgcgaaaa atatcgccac
      841 tggcaaggaa tgggtttgag atggcgaagt ctgtcctaaa agcagcgcct gtagttgtag
      901 ggttgacggc cttgatggag cgtcatgccg atgccctctc gagccaactt caagcacatc
      961 atcttaaggt tttcccgccg cattccgaga agggcattcg aacattcgg
//
